Decidual neutrophil infiltration is not required for preterm birth in a mouse model of infection-induced preterm labor.
Decidual neutrophil infiltration is not required for preterm birth in a mouse model of infection-induced preterm labor.
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DOI：
10.4049/jimmunol.1302891
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发表时间：
2014-03-01
期刊：
影响因子：
--
通讯作者：
Norman JE
中科院分区：
文献类型：
--
作者：
Rinaldi SF;Catalano RD;Wade J;Rossi AG;Norman JE
分娩与白细胞流入子宫内组织有关;然而，这些白细胞在分娩开始中的确切作用尚不清楚。在妇女和小鼠模型中，子宫胎盘组织的神经元浸润与感染相关性早产（PTL）特别相关。在这项研究中，我们研究了中性粒细胞在感染诱导的PTL小鼠模型中的作用。与接受PBS的对照小鼠相比，在妊娠第17天子宫内给予LPS导致蜕膜中性粒细胞数量增加7倍（p < 0.01; n = 8-11）。我们假设中性粒细胞流入是PTL所必需的，中性粒细胞耗竭将消除早产。为了检验这一假设，在妊娠第16天用抗Gr-1、抗Ly-6 G或适当的IgG对照Ab处理小鼠，然后在第17天用LPS处理，以消耗小鼠的中性粒细胞（n = 6-7）。通过流式细胞术和免疫组织化学证实中性粒细胞成功耗竭。用Gr-1耗竭神经元导致子宫和胎盘IL-1β表达降低（p < 0.05）。Ly-6 G去神经元后子宫IL-1β和TNF-α表达降低（p < 0.05）。然而，中性粒细胞耗竭与抗体没有延迟LPS诱导的早产。总的来说，这些数据表明，蜕膜中性粒细胞浸润不是诱导感染诱导的PTL在小鼠中是必不可少的，但中性粒细胞有助于LPS诱导的炎症反应的子宫胎盘组织。
Parturition is associated with a leukocyte influx into the intrauterine tissues; however, the exact role these leukocytes play in the onset of labor remains unclear. Neutrophil infiltration of the uteroplacental tissues has been particularly associated with infection-associated preterm labor (PTL) in both women and mouse models. In this study, we investigated the role of neutrophils in a mouse model of infection-induced PTL. Intrauterine administration of LPS on day 17 of gestation resulted in a 7-fold increase in the number of decidual neutrophils compared with control mice receiving PBS (p < 0.01; n = 8–11). We hypothesized that neutrophil influx is necessary for PTL and that neutrophil depletion would abolish preterm birth. To test this hypothesis, mice were depleted of neutrophils by treatment with anti–Gr-1, anti–Ly-6G, or the appropriate IgG control Ab on day 16 of gestation prior to LPS on day 17 (n = 6–7). Successful neutrophil depletion was confirmed by flow cytometry and immunohistochemistry. Neutrophil depletion with Gr-1 resulted in reduced uterine and placental Il-1β expression (p < 0.05). Neutrophil depletion with Ly-6G reduced uterine Il-1β and Tnf-α expression (p < 0.05). However, neutrophil depletion with either Ab did not delay LPS-induced preterm birth. Collectively, these data show that decidual neutrophil infiltration is not essential for the induction of infection-induced PTL in the mouse, but that neutrophils contribute to the LPS-induced inflammatory response of the uteroplacental tissues.
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DOI：
10.1136/bmj.e6226
发表时间：
2012-10-09
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Haas DM;Caldwell DM;Kirkpatrick P;McIntosh JJ;Welton NJ
通讯作者：
Welton NJ
影响因子：
9.8
作者：
Murphy, Shaun P.;Hanna, Nazeeh N.;Fast, Loren D.;Shaw, Sunil K.;Berg, Goeran;Padbury, James F.;Romero, Roberto;Sharma, Surendra
通讯作者：
Sharma, Surendra
DOI：
10.1080/713605675
发表时间：
2002-10-01
期刊：
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
影响因子：
--
作者：
Helmig, B R;Romero, R;Uldbjerg, N
通讯作者：
Uldbjerg, N
影响因子：
4.4
作者：
Li, Li-Ping;Fang, Yi-Chuan;Saito, Shigeru
通讯作者：
Saito, Shigeru
影响因子：
2.9
作者：
Diamond, Allaire K.;Sweet, Leigh M.;Phillippe, Mark
通讯作者：
Phillippe, Mark