Non-invasive magnetic resonance imaging in rats for prediction of the fate of grafted kidneys from cardiac death donors.
Non-invasive magnetic resonance imaging in rats for prediction of the fate of grafted kidneys from cardiac death donors.
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DOI：
10.1371/journal.pone.0063573
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kobayashi E
中科院分区：
文献类型：
--
作者：
Kaimori JY;Iwai S;Hatanaka M;Teratani T;Obi Y;Tsuda H;Isaka Y;Yokawa T;Kuroda K;Ichimaru N;Okumi M;Yazawa K;Rakugi H;Nonomura N;Takahara S;Kobayashi E
本研究的主要目的是在移植前评估心脏死亡(CD)肾移植物，以确定血氧水平依赖(BOLD)和弥散磁共振技术是否能预测移植后移植物的损害。我们通过BOLD和弥散磁共振成像对Cd肾组织进行评估。我们还检测了移植前后CD肾的病理和基因表达的变化。与CD0h、CD2 h相比，移植肾移植前CD1h和CD2 h移植肾外髓质内纹区红细胞充血(RCC)明显增多(p<0.0 5)，但移植肾移植后2 d外延髓内纹区红细胞充血(RCC)明显增加(p<0.0 5)。与病理结果一致的是，移植肾组织纤溶酶原激活物(TPA)基因仅在移植肾皮质和髓质表达增加。在CD1h和CD2h移植肾中，BOLD MRI成功地、非侵入性地成像和量化了IS中的RCC(p<；0.05)。弥散磁共振成像也无创评估增加表观弥散系数在移植物CD2小时(OS)，减少它在外条(OS)，与IS的间质水肿和OS的小管细胞水肿相一致。外髓的这两种类型的水肿可以解释仅CD2小时移植肾的IS中RCC延长的原因，这是抑制IS中毛细血管出来的红细胞的恶性循环的一部分。在MRI测量前用威斯康星大学溶液灌流并不能减少CD1h和CD2h移植肾之间的组织损伤差异。BOLD和弥散磁共振成像是评估Cd移植肾组织损伤的有效无创性工具，可以预测移植Cd移植肾的长期器官损伤，从而预测移植肾的预后。
The main objective of this study was to assess cardiac death (CD) kidney grafts before transplantation to determine whether blood oxygen level-dependent (BOLD) and diffusion MRI techniques can predict damage to these grafts after transplantation. We assessed CD kidney tissue by BOLD and diffusion MRI. We also examined pathological and gene expression changes in CD kidney grafts before and after transplantation. Although there was significantly more red cell congestion (RCC) in the inner stripe of the outer medulla (IS) in both 1 h after cardiac death (CD1h) and CD2h kidneys destined for grafts before transplantation compared with CD0h (p<0.05), CD2h, but not CD1h, kidney grafts had significantly different RCC in the IS 2 days after transplantation (p<0.05). Consistent with these pathological findings, tissue plasminogen activator (tPA) gene expression was increased only in the cortex and medulla of CD2h kidney grafts after transplantation. BOLD MRI successfully and non-invasively imaged and quantified RCC in the IS in both CD1h and CD2h kidney grafts (p<0.05). Diffusion MRI also non-invasively assessed increased the apparent diffusion coefficient in the IS and decreased it in the outer stripe (OS) of CD2h grafts, in concordance with interstitial edema in the IS and tubule cellular edema in the OS. These two types of edema in the outer medulla could explain the prolonged RCC in the IS only of CD2h kidney grafts, creating part of a vicious cycle inhibiting red cells coming out of capillary vessels in the IS. Perfusion with University of Wisconsin solution before MRI measurements did not diminish the difference in tissue damage between CD1h and CD2h kidney grafts. BOLD and diffusion MRI, which are readily available non-invasive tools for evaluating CD kidney grafts tissue damage, can predict prolonged organ damage, and therefore the outcome, of transplanted CD kidney grafts.
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影响因子：
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作者：
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