Proteomic Alterations in Aqueous Humor From Patients With Primary Open Angle Glaucoma.
Proteomic Alterations in Aqueous Humor From Patients With Primary Open Angle Glaucoma.
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DOI：
10.1167/iovs.17-23434
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发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Sharma A
中科院分区：
文献类型：
--
作者：
Sharma S;Bollinger KE;Kodeboyina SK;Zhi W;Patton J;Bai S;Edwards B;Ulrich L;Bogorad D;Sharma A
原发性开角型青光眼(POAG)是最常见的青光眼类型，约占所有青光眼病例的90%。房水是一种存在于眼的前房和后房的生物液体，参与多种功能，包括维持眼压和眼内环境的稳定。这种液体非常接近病理部位，也被认为在青光眼发病机制中起着重要作用。本研究的目的是确定开角型青光眼患者的蛋白组学改变。取自47例白内障手术患者(对照组：32例；开角型青光眼：15例)。用液-质联用技术对消化后的样品进行蛋白质组学分析。使用各种统计和生物信息学方法对鉴定出的蛋白质进行评估。与对照组相比，POAG受试者共有33种蛋白质发生了显著改变。POAG患者中最丰富的蛋白质是IGKC(13.56倍)、ITIH4(4.1倍)、APOC3(3.36倍)、IDH3A(3.11倍)、LOC105369216(2.98倍)。SERPINF2(2.94倍)、NPC2(2.88倍)、SUCLG2(2.70倍)、KIAA0100(2.29倍)、CNOT4(2.23倍)、AQP4(2.11倍)、COL18A1(2.08倍)、NWD1(2.07倍)和TMEM120B(2.06倍)。随着这些蛋白质水平的增加，发生POAG的优势比有显著的增加趋势。在这项研究中确定的蛋白质与信号、糖基化、免疫反应、分子运输和脂质代谢有关。识别的候选蛋白可能是与POAG发生相关的潜在生物标志物，并可能有助于更深入地了解该病的发病机制。
Primary open angle glaucoma (POAG) is the most prevalent form of glaucoma, accounting for approximately 90% of all cases. The aqueous humor (AH), a biological fluid in the anterior and posterior chambers of the eye, is involved in a multitude of functions including the maintenance of IOP and ocular homeostasis. This fluid is very close to the pathologic site and is also known to have a significant role in glaucoma pathogenesis. The purpose of this study was to identify proteomic alterations in AH from patients with POAG. AH samples were extracted from 47 patients undergoing cataract surgery (controls: n = 32; POAG: n = 15). Proteomic analysis of the digested samples was accomplished by liquid-chromatography-mass spectrometry. The identified proteins were evaluated using a variety of statistical and bioinformatics methods. A total of 33 proteins were significantly altered in POAG subjects compared with the controls. The most abundant proteins in POAG subjects are IGKC (13.56-fold), ITIH4 (4.1-fold), APOC3 (3.36-fold), IDH3A (3.11-fold), LOC105369216 (2.98-fold). SERPINF2 (2.94-fold), NPC2 (2.88-fold), SUCLG2 (2.70-fold), KIAA0100 (2.29-fold), CNOT4 (2.23-fold), AQP4 (2.11-fold), COL18A1 (2.08-fold), NWD1 (2.07-fold), and TMEM120B (2.06-fold). A significant increasing trend in the odds ratios of having POAG was observed with increased levels of these proteins. Proteins identified in this study are implicated in signaling, glycosylation, immune response, molecular transport, and lipid metabolism. The identified candidate proteins may be potential biomarkers associated with POAG development and may lead to more insight in understanding the mechanisms underlying the pathogenesis of this disease.
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影响因子：
17.8
作者：
Janssen, Sarah F.;Gorgels, Theo G. M. F.;Bergen, Arthur A. B.
通讯作者：
Bergen, Arthur A. B.
影响因子：
5
作者：
Kashyap, Rajpal S.;Nayak, Amit R.;Daginawala, Hatim F.
通讯作者：
Daginawala, Hatim F.
影响因子：
9.8
作者：
Jiao, XD;Ventruto, V;Hejtmancik, JF
通讯作者：
Hejtmancik, JF
影响因子：
4.4
作者：
Chowdhury, Uttio Roy;Madden, Benjamin J.;Fautsch, Michael P.
通讯作者：
Fautsch, Michael P.
DOI：
10.1590/s0001-37652006000100012
发表时间：
2006-03-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
Passos-Bueno, Maria Rita;Suzuki, Oscar T.;Leite, Katia R.M.
通讯作者：
Leite, Katia R.M.