Anticancer activity of marine sponge Hyrtios sp. extract in human colorectal carcinoma RKO cells with different p53 status.
Anticancer activity of marine sponge Hyrtios sp. extract in human colorectal carcinoma RKO cells with different p53 status.
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DOI：
10.1155/2014/413575
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发表时间：
2014
影响因子：
--
通讯作者：
Jung J
中科院分区：
文献类型：
--
作者：
Lim HK;Bae W;Lee HS;Jung J
利用海洋生物资源进行药物开发是有限的，尽管海洋占据了地球的70%左右，并含有大量的生物材料。从海绵提取物的筛选试验中，我们发现了Hyrtios sp.采集自密克罗尼西亚丘克岛的海绵。在本研究中，Hyrtios sp.提取物对野生型P53和P53缺陷的RKO-E6细胞的抗癌活性进行了检测。Hyrtios sp.提取物呈剂量依赖性抑制两种细胞系的存活率。多核也被观察到是有丝分裂灾难的迹象。细胞毒性试验结果显示，海藻作用48 h后，RKO和RKO-E6细胞的毒性结果有显著差异。提取。在用Hyrtios sp.处理的RKO细胞中。提取物诱导细胞死亡是通过诱导p53和p21蛋白表达来实现的。在p53基因缺陷的RKO-E6细胞中，Hyrtios sp.提取物降低JNK蛋白表达，上调p21蛋白表达。这些结果表明，Hyrtios sp.提取物通过不同的途径诱导细胞凋亡，这取决于P53的状态，可能是开发新的抗癌药物的良好天然产物。
Drug development using marine bioresources is limited even though the ocean occupies about 70% of the earth and contains a large number of biological materials. From the screening test of the marine sponge extracts, we found Hyrtios sp. sponge collected from Chuuk island, Micronesia. In this study, the Hyrtios sp. extract was examined for anticancer activity against human colorectal carcinoma RKO cells that are wildtype for p53 and RKO-E6 that are p53 defective. The Hyrtios sp. extract dose-dependently inhibited viability in both cell lines. Multinucleation as an indication of mitotic catastrophe was also observed. Cytotoxicity tests gave significantly different results for RKO and RKO-E6 cells after 48 h exposure to Hyrtios sp. extract. In RKO cells treated with Hyrtios sp. extract, cell death occurred by induction of p53 and p21 proteins. In p53-defective RKO-E6 cells, Hyrtios sp. extract decreased expression of JNK protein and increased p21 protein. These results indicate that Hyrtios sp. extract induced apoptosis via different pathways depending on p53 status and could be a good natural product for developing new anticancer drugs.
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
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5.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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