Acoel single-cell atlas reveals expression dynamics and heterogeneity of adult pluripotent stem cells.
Acoel single-cell atlas reveals expression dynamics and heterogeneity of adult pluripotent stem cells.
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DOI：
10.1038/s41467-023-38016-4
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发表时间：
2023-05-05
影响因子：
16.6
通讯作者：
Srivastava, Mansi
中科院分区：
文献类型：
--
作者：
Hulett, Ryan E.;Kimura, Julian O.;Bolanos, D. Marcela;Luo, Yi-Jyun;Rivera-Lopez, Carlos;Ricci, Lorenzo;Srivastava, Mansi
成体多能干细胞 (aPSC) 群体是许多远缘动物谱系全身再生的基础，但不同物种之间的潜在细胞和分子机制如何比较尚不清楚。在这里，我们应用单细胞 RNA 测序来分析无腔蠕虫 Hofstenia miamia 在胚胎后发育和再生过程中的转录细胞状态。我们确定了再生过程中跨阶段共享的细胞类型及其相关的基因表达动态。功能研究证实，aPSC（也称为新生细胞）是分化细胞的来源，并揭示了分化所需的转录因子。新生细胞的亚群恢复了转录上不同的亚群，其中大多数可能专门针对分化的谱系。一个新生细胞亚群显示出组蛋白变体 H3.3 的丰富表达，但似乎缺乏特化。总而言之，本研究中确定的细胞状态有助于与其他物种进行比较，并使未来对干细胞命运潜力的研究成为可能。多个物种可以通过成体干细胞的作用实现全身再生，但人们对这些细胞在物种间的比较情况知之甚少。在这里，他们研究了海洋蠕虫 acoel Hofstenia miamia 的干细胞，并鉴定了这些细胞在发育和再生过程中的异质性。
Adult pluripotent stem cell (aPSC) populations underlie whole-body regeneration in many distantly-related animal lineages, but how the underlying cellular and molecular mechanisms compare across species is unknown. Here, we apply single-cell RNA sequencing to profile transcriptional cell states of the acoel worm Hofstenia miamia during postembryonic development and regeneration. We identify cell types shared across stages and their associated gene expression dynamics during regeneration. Functional studies confirm that the aPSCs, also known as neoblasts, are the source of differentiated cells and reveal transcription factors needed for differentiation. Subclustering of neoblasts recovers transcriptionally distinct subpopulations, the majority of which are likely specialized to differentiated lineages. One neoblast subset, showing enriched expression of the histone variant H3.3, appears to lack specialization. Altogether, the cell states identified in this study facilitate comparisons to other species and enable future studies of stem cell fate potentials. Multiple species can achieve whole-body regeneration through the action of adult stem cells, though little is known about how these cells compare across species. Here they investigated the stem cells of a marine worm, the acoel Hofstenia miamia, and identified heterogeneity of these cells during development and regeneration.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
64.5
作者：
Goldberg AD;Banaszynski LA;Noh KM;Lewis PW;Elsaesser SJ;Stadler S;Dewell S;Law M;Guo X;Li X;Wen D;Chapgier A;DeKelver RC;Miller JC;Lee YL;Boydston EA;Holmes MC;Gregory PD;Greally JM;Rafii S;Yang C;Scambler PJ;Garrick D;Gibbons RJ;Higgs DR;Cristea IM;Urnov FD;Zheng D;Allis CD
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Allis CD
影响因子：
4
作者：
Gahan, James M.;Bradshaw, Brian;Frank, Uri
通讯作者：
Frank, Uri
HTZ-1/H2A.Z 和 PHA-4/FoxA 对前肠发育的时间调节。
影响因子：
4.5
作者：
Updike, Dustin L.;Mango, Susan E.
通讯作者：
Mango, Susan E.
影响因子：
10.7
作者：
Duruz J;Kaltenrieder C;Ladurner P;Bruggmann R;Martìnez P;Sprecher SG
通讯作者：
Sprecher SG