HrcT Is a Key Component of the Type III Secretion System in Xanthomonas spp. and Also Regulates the Expression of the Key hrp Transcriptional Activator HrpX
HrcT Is a Key Component of the Type III Secretion System in Xanthomonas spp. and Also Regulates the Expression of the Key hrp Transcriptional Activator HrpX
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HrcT 是黄单胞菌属 III 型分泌系统的关键组成部分。
DOI：
10.1128/aem.00308-14
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发表时间：
2014-04
影响因子：
4.4
通讯作者：
Chen Gong-You
中科院分区：
文献类型：
--
作者：
Liu Zhi-Yang;Zou Li-Fang;Xue Xiao-Bo;Cai Lu-Lu;Ma Wen-Xiu;Xiong Li;Ji Zhi-Yuan;Chen Gong-You
ABSTRACT The type III secretion system (T3SS), encoded by hrp (hypersensitive response and pathogenicity) genes in Gram-negative phytopathogenic bacteria, delivers repertoires of T3SS effectors (T3SEs) into plant cells to trigger the hypersensitive response (HR) in nonhost or resistant-host plants and promote pathogenicity in susceptible plants. The expression of hrp genes in Xanthomonas is regulated by two key regulatory proteins, HrpG and HrpX. However, the interactions between hrp gene products in directing T3SE secretion are largely unknown. Here we demonstrated that HrcT of X. oryzae pv. oryzicola functions as a T3SS component and positively regulates the expression of hrpX. Transcription of hrcT occurs via two distinct promoters; one (T1) is with the hrpB operon and the second (T3) within hrpB7 Via either promoter T1 or T3, the defect in Hrp phenotype by hrcT deletion was corrected in the presence of hrcT only from Xanthomonas species but not from other phytopathogenic bacteria. An N-terminally truncated HrcT was able to bind the hrpX promoter and activate the expression of hrpX, supporting that HrcT is a positive regulator of hrpX. A revised model showing the regulatory interactions between HrcT, HrpX, and HrpG is proposed.
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影响因子：
1.5
作者：
Huasong Zou;Xue Song;Lifang Zou;Liang Yuan;Yu-rong Li;W. Guo;Y. Che;Wenxiang Zhao;Y. Duan
通讯作者：
Huasong Zou;Xue Song;Lifang Zou;Liang Yuan;Yu-rong Li;W. Guo;Y. Che;Wenxiang Zhao;Y. Duan
影响因子：
2.1
作者：
Furutani, A;Nakayama, T;Tsuge, S
通讯作者：
Tsuge, S
影响因子：
3.2
作者：
Yingxian Xiao;S. Heu;J. Yi;Y. Lu;S. Hutcheson
通讯作者：
Yingxian Xiao;S. Heu;J. Yi;Y. Lu;S. Hutcheson
HrpD6 在调节米黄单胞菌 hrp-hrc-hpa 基因中的新调控作用。
影响因子：
3.5
作者：
Li, Yu-Rong;Zou, Hua-Song;Chen, Gong-You
通讯作者：
Chen, Gong-You
影响因子：
4.4
作者：
Lifang Zou;Xingsheng Wang;Y. Xiang;Bing Zhang;Yu-rong Li;You-lun Xiao;Jin-sheng Wang;A. Walmsley-A.-Walms
通讯作者：
Lifang Zou;Xingsheng Wang;Y. Xiang;Bing Zhang;Yu-rong Li;You-lun Xiao;Jin-sheng Wang;A. Walmsley-A.-Walms