Mapping the cellular electrophysiology of rat sympathetic preganglionic neurones to their roles in cardiorespiratory reflex integration: a whole cell recording study in situ.
Mapping the cellular electrophysiology of rat sympathetic preganglionic neurones to their roles in cardiorespiratory reflex integration: a whole cell recording study in situ.
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DOI：
10.1113/jphysiol.2014.270769
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发表时间：
2014-05-15
期刊：
影响因子：
--
通讯作者：
Pickering AE
中科院分区：
文献类型：
--
作者：
Stalbovskiy AO;Briant LJ;Paton JF;Pickering AE
交感神经节前神经元（spn）将交感神经活动从中枢神经系统传递到外周到达目标器官。尽管之前的体内和体外细胞记录研究已经探索了它们的电生理特征，但还不可能将这些特征与它们在心肺反射整合中的作用联系起来。我们使用工作的心-脑干制剂对T3-4 spn进行全细胞膜片钳记录（n = 98）。这些spn根据其对外周化学反射、潜水反应和动脉压力反射的不同反应进行分类，从而可以区分肌肉血管收缩样（mvc样，39%）和皮肤血管收缩样（cvc样，28%）spn。mvc样spn具有更高的基线发射频率（2.52±0.33 Hz vs. cvc样1.34±0.17 Hz, P = 0.007）。cvc样具有较长的后超极化（314±36 ms， mvc样为191±13 ms， P < 0.001）和较低的输入电阻（346±49 MΩ， mvc样为496±41 MΩ， P < 0.05）。mvc样放电是呼吸调节的，在吸气晚期/呼气早期有峰值放电，这种活动是由张力和呼吸调节的突触事件弹幕产生的，这些突触事件被鞘内肌酸阻断。相反，cvc样spn的活性是由提示间隙连接耦合的节律性膜电位振荡支撑的。因此，我们将两类spn的固有电生理特性与其在心肺反射整合中的作用联系起来，并表明它们部署不同的细胞机制，可能影响它们如何整合和形成独特的交感输出。
Sympathetic preganglionic neurones (SPNs) convey sympathetic activity flowing from the CNS to the periphery to reach the target organs. Although previous in vivo and in vitro cell recording studies have explored their electrophysiological characteristics, it has not been possible to relate these characteristics to their roles in cardiorespiratory reflex integration. We used the working heart–brainstem preparation to make whole cell patch clamp recordings from T3–4 SPNs (n = 98). These SPNs were classified by their distinct responses to activation of the peripheral chemoreflex, diving response and arterial baroreflex, allowing the discrimination of muscle vasoconstrictor-like (MVClike, 39%) from cutaneous vasoconstrictor-like (CVClike, 28%) SPNs. The MVClike SPNs have higher baseline firing frequencies (2.52 ± 0.33 Hz vs. CVClike 1.34 ± 0.17 Hz, P = 0.007). The CVClike have longer after-hyperpolarisations (314 ± 36 ms vs. MVClike 191 ± 13 ms, P < 0.001) and lower input resistance (346 ± 49 MΩ vs. MVClike 496 ± 41 MΩ, P < 0.05). MVClike firing was respiratory-modulated with peak discharge in the late inspiratory/early expiratory phase and this activity was generated by both a tonic and respiratory-modulated barrage of synaptic events that were blocked by intrathecal kynurenate. In contrast, the activity of CVClike SPNs was underpinned by rhythmical membrane potential oscillations suggestive of gap junctional coupling. Thus, we have related the intrinsic electrophysiological properties of two classes of SPNs in situ to their roles in cardiorespiratory reflex integration and have shown that they deploy different cellular mechanisms that are likely to influence how they integrate and shape the distinctive sympathetic outputs.
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