Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status.
Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA) binds human erythrocytes independent of Duffy antigen status.
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DOI：
10.1038/srep35581
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发表时间：
2016-10-19
影响因子：
4.6
通讯作者：
Han ET
中科院分区：
文献类型：
--
作者：
Cheng Y;Lu F;Wang B;Li J;Han JH;Ito D;Kong DH;Jiang L;Wu J;Ha KS;Takashima E;Sattabongkot J;Cao J;Nyunt MH;Kyaw MP;Desai SA;Miller LH;Tsuboi T;Han ET
间日疟原虫是温带和热带气候下疟疾的主要病原体，人们认为它无法感染缺乏达菲（Fy）血型抗原的人类，因为这种受体对于红细胞入侵至关重要。然而，最近在各流行地区的调查报告了达菲阴性个体中的间日疟原虫感染，表明寄生虫可能利用替代受体-配体对来完成红细胞侵入。在这里，我们确定了一种新的寄生虫配体，间日疟原虫GPI锚定的微线抗原（PvGAMA），结合人类红细胞，无论达菲抗原状态。PvGAMA定位在成熟的寄生虫期的微线。针对PvGAMA片段的抗体以剂量依赖性方式抑制PvGAMA与红细胞的结合。糜蛋白酶处理后PvGAMA的红细胞特异性结合活性显著降低。因此，PvGAMA可能是一种粘附分子的入侵达菲阳性和阴性的人红细胞。
Plasmodium vivax, a major agent of malaria in both temperate and tropical climates, has been thought to be unable to infect humans lacking the Duffy (Fy) blood group antigen because this receptor is critical for erythrocyte invasion. Recent surveys in various endemic regions, however, have reported P. vivax infections in Duffy-negative individuals, suggesting that the parasite may utilize alternative receptor-ligand pairs to complete the erythrocyte invasion. Here, we identified and characterized a novel parasite ligand, Plasmodium vivax GPI-anchored micronemal antigen (PvGAMA), that bound human erythrocytes regardless of Duffy antigen status. PvGAMA was localized at the microneme in the mature schizont-stage parasites. The antibodies against PvGAMA fragments inhibited PvGAMA binding to erythrocytes in a dose-dependent manner. The erythrocyte-specific binding activities of PvGAMA were significantly reduced by chymotrypsin treatment. Thus, PvGAMA may be an adhesion molecule for the invasion of Duffy-positive and -negative human erythrocytes.
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影响因子：
3.8
作者：
Hostetler JB;Lo E;Kanjee U;Amaratunga C;Suon S;Sreng S;Mao S;Yewhalaw D;Mascarenhas A;Kwiatkowski DP;Ferreira MU;Rathod PK;Yan G;Fairhurst RM;Duraisingh MT;Rayner JC
通讯作者：
Rayner JC
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影响因子：
4.6
作者：
Han JH;Lee SK;Wang B;Muh F;Nyunt MH;Na S;Ha KS;Hong SH;Park WS;Sattabongkot J;Tsuboi T;Han ET
通讯作者：
Han ET
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Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
3.8
作者：
Hostetler JB;Sharma S;Bartholdson SJ;Wright GJ;Fairhurst RM;Rayner JC
通讯作者：
Rayner JC
影响因子：
7
作者：
Gilson, Paul R.;Nebl, Thomas;Crabb, Brendan S.
通讯作者：
Crabb, Brendan S.