The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration.
The Cx43-like connexin protein Cx40.8 is differentially localized during fin ontogeny and fin regeneration.
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DOI：
10.1371/journal.pone.0031364
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Iovine MK
中科院分区：
文献类型：
--
作者：
Gerhart SV;Eble DM;Burger RM;Oline SN;Vacaru A;Sadler KC;Jefferis R;Iovine MK
连接蛋白（Cx）是间隙连接的亚基，间隙连接是允许相邻细胞之间小分子交换的膜蛋白通道。Cx43是斑马鱼尾鳍细胞增殖所必需的。以前，我们发现，Cx43样连接蛋白，cx40.8，与cx43在鳍再生过程中的增殖细胞的人口共表达。在这里，我们表明，Cx40.8表现出新的差异亚细胞定位在体内，这取决于鳍的生长状态。在鳍的个体发育过程中，Cx40.8被发现在质膜，但Cx40.8是保留在高尔基体在再生过程中。接下来，我们鉴定了Cx40.8的30个氨基酸结构域，其负责其动态定位。差异定位的一个可能的解释是，Cx40.8有助于调节Cx43在体内，也许修改通道活动在个体发育的增长。然而，我们发现Cx40.8的电压门控特性与Cx43相似。总之，我们的研究结果表明，Cx40.8在体内表现出不同的亚细胞定位，依赖于其羧基末端的离散域。我们认为，Cx40.8的动态定位差异影响Cx43依赖的细胞增殖过程中的个体发育和再生。
Connexins (Cx) are the subunits of gap junctions, membraneous protein channels that permit the exchange of small molecules between adjacent cells. Cx43 is required for cell proliferation in the zebrafish caudal fin. Previously, we found that a Cx43-like connexin, cx40.8, is co-expressed with cx43 in the population of proliferating cells during fin regeneration. Here we demonstrate that Cx40.8 exhibits novel differential subcellular localization in vivo, depending on the growth status of the fin. During fin ontogeny, Cx40.8 is found at the plasma membrane, but Cx40.8 is retained in the Golgi apparatus during regeneration. We next identified a 30 amino acid domain of Cx40.8 responsible for its dynamic localization. One possible explanation for the differential localization is that Cx40.8 contributes to the regulation of Cx43 in vivo, perhaps modifying channel activity during ontogenetic growth. However, we find that the voltage-gating properties of Cx40.8 are similar to Cx43. Together our findings reveal that Cx40.8 exhibits differential subcellular localization in vivo, dependent on a discrete domain in its carboxy terminus. We suggest that the dynamic localization of Cx40.8 differentially influences Cx43-dependent cell proliferation during ontogeny and regeneration.
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DOI：
10.1083/jcb.129.3.805
发表时间：
1995-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Elfgang C;Eckert R;Lichtenberg-Fraté H;Butterweck A;Traub O;Klein RA;Hülser DF;Willecke K
通讯作者：
Willecke K
影响因子：
4.5
作者：
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通讯作者：
Pointis, Georges
影响因子：
4
作者：
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通讯作者：
Pointis, Georges
影响因子：
3.5
作者：
Lauf, U;Lopez, P;Falk, MM
通讯作者：
Falk, MM
影响因子：
2.7
作者：
Sims, Kenneth, Jr.;Eble, Diane M.;Iovine, M. Kathryn
通讯作者：
Iovine, M. Kathryn