Myosin Vc, a Member of the Actin Motor Family Associated with Rab8, Is Involved in the Release of DV2 from HepG2 Cells
Myosin Vc, a Member of the Actin Motor Family Associated with Rab8, Is Involved in the Release of DV2 from HepG2 Cells
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肌球蛋白 Vc 是与 Rab8 相关的肌动蛋白运动家族成员，参与 HepG2 细胞释放 DV2
作者：
目的：登革热病毒（DV）感染的发病机制尚未明确。我们已经报道肌动蛋白和 Rab8 参与 DV2 感染。肌球蛋白 Vc (Myo5c) 是 V 类肌球蛋白的新成员，调节与 Rab8 相关的肌动蛋白介导的膜运输。在本研究中，在 HpeG2 细胞中研究了 Myo5c 参与 DV2 释放的情况。方法：通过荧光染色揭示肌动蛋白、Myo5c、DV2 和 Rab8 的分布。通过转染构建表达 Myo5c 显性失活突变体的 HepG2Myo5c-tail 细胞，并通过蛋白质印迹进行评估。采用噬菌斑测定法检测病毒滴度，流式细胞术分析Rab8的表达。结果：DV2感染改变了Myo5c的分布模式，这可能与肌动蛋白的解聚有关，尽管Myo5c与DV2或肌动蛋白的共定位率较低。此外，HepG2Myo5c-tail 细胞中 DV2 的释放减少，但细胞内病毒的产生并未减少。此外，Myo5c 与 Rab8 共定位，并且 Rab8 的增加与 Myo5c 尾部引起的病毒释放减少相关。结论：我们的数据表明与 Rab8 相关的 Myo5c 参与了 HepG2 细胞的 DV2 释放。
Objective: The pathogenesis of the dengue virus (DV) infection has not been well defined. We have reported that actin and Rab8 are involved in DV2 infection. Myosin Vc (Myo5c) is a novel member of the class V myosins and regulates the actin-mediated membrane trafficking associated with Rab8. In this study, the involvement of Myo5c in the release of DV2 was investigated in HpeG2 cells. Methods: Distributions of actin, Myo5c, DV2 and Rab8 were revealed by fluorescent staining. HepG2Myo5c-tail cells expressing a dominant-negative mutant of Myo5c were constructed by transfection and were assessed by Western blotting. The viral titers were detected by plaque assay, and the expression of Rab8 was analyzed by flow cytometry. Results: DV2 infection altered the distribution pattern of Myo5c, which might be associated with the depolymerization of actin, though colocalization rates of Myo5c with DV2 or actin were low. Furthermore, the release of DV2, but not the intracellular viral production, was reduced from HepG2Myo5c-tail cells. Moreover, Myo5c colocalized with Rab8 and an increase of Rab8 was associated with the decrease of the viral release caused by the Myo5c tail.Conclusions: Our data suggest that Myo5c associated with Rab8 is involved in the release of DV2 from HepG2 cells.
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DOI：
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发表时间：
2005
期刊：
Chinese journal of zoonoses
影响因子：
--
作者：
An Jing
通讯作者：
An Jing
DOI：
10.1073/pnas.2434327100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Brock, SC;Goldenring, JR;Crowe, JE
通讯作者：
Crowe, JE
影响因子：
4
作者：
Hattula, Katarina;Furuhjelm, Johanna;Peranen, Johan
通讯作者：
Peranen, Johan
DOI：
10.4269/ajtmh.1992.47.265
发表时间：
1992-09-01
影响因子：
3.3
作者：
KUO, CH;TAI, DI;LIAW, YF
通讯作者：
LIAW, YF
影响因子：
4.6
作者：
Xiaofeng Xu;Zong-tao Chen;Jun-lei Zhang;Wei Chen;Jia-li Wang;Yan-Ping Tian;Na Gao;J. An
通讯作者：
Xiaofeng Xu;Zong-tao Chen;Jun-lei Zhang;Wei Chen;Jia-li Wang;Yan-Ping Tian;Na Gao;J. An