Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax
Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax
复制标题
微生物木聚糖分解活性对芦竹根茎半纤维素的降解作用
作者：
L. Lama;A. Tramice;I. Finore;Gianluca Anzelmo;V. Calandrelli;E. Pagnotta;G. Tommonaro;A. Poli;P. Di Donato;B. Nicolaus;M. Fagnano;M. Mori;A. Impagliazzo;A. Trincone
极端细菌产生的多糖酶具有独特的作用、对不同反应条件的耐受性以及其他一些生物技术上感兴趣的特性。本文研究了嗜热微生物(Thermotoga Neapolitana)、嗜热地杆菌和嗜热厌氧杆菌粗提物对最有价值的生物质作物--芦苇(Arundo donax L.)半纤维素的作用。这种生物量可以在没有竞争的情况下种植，在种植周期结束时(10-15年)，大量的根茎留在土壤中，代表着另一个有用分子的来源。研究了从杜鹃根茎中提取半纤维素组分的工艺条件。从嗜热微生物粗提物中提取的多糖酶被证明适合于从半纤维素中完全降解和/或生产有用的小寡糖。木二糖和感兴趣的四糖和五糖是在不同的条件下通过酶作用得到的。每毫克粗酶可加工适量的原料。与分离的四糖和五糖不同，原半纤维素和经过处理的材料显示出抗氧化活性。结果表明，根茎是生产有价值的工业产品的有用原料，因此可以提高冬虫夏草栽培的经济效益。
Polysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana, Geobacillus thermantarcticus and Thermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donax L.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10–15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes of Arundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses from A. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency of A. donax cultivation.
影响因子：
2.7
作者：
MEYER, BN;FERRIGNI, NR;MCLAUGHLIN, JL
通讯作者：
MCLAUGHLIN, JL