Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies
Detection of herpes simplex virus in conventional tube cell cultures and in shell vials with a DNA probe kit and monoclonal antibodies
复制标题
使用 DNA 探针试剂盒和单克隆抗体检测传统管细胞培养物和壳瓶中的单纯疱疹病毒
DOI：
10.1128/jcm.26.1.22-24.1988
复制
发表时间：
1988
影响因子：
9.4
通讯作者：
Thomas F. Smith
中科院分区：
文献类型：
--
作者：
M. Espy;Thomas F. Smith
将提交用于诊断单纯疱疹病毒（HSV）感染的样本接种到带壳小瓶中，并与商业DNA探针试剂盒（Pathogene; Enzo Biochem，Inc.，纽约州纽约）接种后16 h进行免疫荧光检测。结果进行了比较，在传统的试管细胞培养的病毒分离。在504份标本中，105份（20.8%）为HSV阳性。在105份标本中，所有3种检测系统均检出93份HSV阳性标本（89%）。与常规试管细胞培养中的病毒回收率（识别细胞病变效应的平均时间，2天）相比，通过壳瓶中的探针或单克隆抗体试验获得HSV的最大检出率（100/105 [95%]），其灵敏度分别为98%和97%。两种壳小瓶试验的特异性均为99%。DNA探针试剂盒可用作单克隆抗体和荧光测定法的替代方法，作为快速实验室检测HSV感染的诊断方法。
Specimens submitted for diagnosis of herpes simplex virus (HSV) infection were inoculated into shell vials and reacted with a commercial DNA probe kit (Pathogene; Enzo Biochem, Inc., New York, N.Y.) and an immunofluorescence assay at 16 h postinoculation. The results were compared with isolation of the virus in conventional tube cell cultures. Of 504 specimens, 105 (20.8%) were positive for HSV. Of the 105, 93 HSV-positive specimens (89%) were detected by all three assay systems. Maximum detection of HSV (100 of 105 [95%]) was obtained by probe or monoclonal antibody assay in shell vials, which had sensitivities of 98 and 97%, respectively, compared with viral recovery in conventional tube cell cultures (mean time for recognition of cytopathic effects, 2 days). Both shell vial assays were 99% specific. The DNA probe kit may be used as an alternative to a monoclonal antibody and fluorescence assay in shell vials as a diagnostic method for rapid laboratory detection of HSV infection.
DOI：
10.1093/infdis/150.2.272
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Myerson,D;Hackman,RC;Meyers,JD
通讯作者：
Meyers,JD