The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury.
The effect of transient oxygenation on stem cell mobilization and ischemia/reperfusion heart injury.
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DOI：
10.1371/journal.pone.0192733
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Li TS
中科院分区：
文献类型：
--
作者：
Yano R;Inadomi C;Luo L;Goto S;Hara T;Li TS
对于全身麻醉，在插管前常规进行预氧合。众所周知，缺血/缺氧预处理诱导干细胞动员并保护缺血/再灌注（I/R）损伤。在这项研究中，我们研究了短暂氧合对干细胞动员和心脏I/R损伤的影响。将小鼠暴露于100%氧气中5或20分钟。我们评估了氧合后1、3、6和24小时外周血中c-kit+干/祖细胞的数量以及SDF-1α和VEGF的水平。我们还在氧合后3小时诱导心脏I/R损伤5分钟，然后在3或14天后检查心脏中的干细胞募集和纤维化变化。在氧合5或20分钟后1或24小时，外周血中c-kit+细胞的数量显著增加。氧合5或20分钟未显著改变血浆中测定的SDF-1α水平。然而，血浆VEGF水平在氧合后3小时降低20分钟（p = 0.051）。氧合5分钟没有显著改变纤维化面积或细胞凋亡。虽然氧合5分钟增加了I/R损伤心脏中c-kit+细胞的数量，但由于个体之间的差异很大，因此组间差异不显著（p = 0.14）。虽然短暂的氧合诱导干细胞动员，但它似乎并不能保护小鼠心脏免受I/R损伤。
For general anesthesia, pre-oxygenation is routinely performed prior to intubation. It is well-known that ischemic/hypoxic preconditioning induces stem cell mobilization and protects against ischemia/reperfusion (I/R) injury. In this study, we investigated the effect of transient oxygenation on stem cell mobilization and I/R injury of the heart. Mice were exposed to 100% oxygen for 5 or 20 minutes. We evaluated the number of c-kit+ stem/progenitor cells and the levels of SDF-1α and VEGF in peripheral blood at 1, 3, 6, and 24 hours after oxygenation. We also induced I/R injury of the heart at 3 hours post-oxygenation for 5 minutes and then examined stem cell recruitment and fibrotic changes in the heart 3 or 14 days later. The number of c-kit+ cells in peripheral blood was significantly increased at 1 or 24 hours after oxygenation for either 5 or 20 minutes. Oxygenation for 5 or 20 minutes did not significantly change the SDF-1α level measured in plasma. However, the plasma VEGF level was decreased at 3 hours post-oxygenation for 20 minutes (p = 0.051). Oxygenation for 5 minutes did not significantly alter the fibrotic area or cell apoptosis. Although oxygenation for 5 minutes increased the number of c-kit+ cells in hearts damaged by I/R injury, this difference was not significant between groups due to large variation between individuals (p = 0.14). Although transient oxygenation induces stem cell mobilization, it does not appear to protect against I/R injury of the heart in mice.
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影响因子：
37.8
作者：
MURRY, CE;JENNINGS, RB;REIMER, KA
通讯作者：
REIMER, KA
影响因子：
24
作者：
Kamota, Takahiro;Li, Tao-Sheng;Hamano, Kimikazu
通讯作者：
Hamano, Kimikazu
DOI：
10.4081/ejh.2013.e14
发表时间：
2013-05-09
期刊：
European journal of histochemistry : EJH
影响因子：
--
作者：
Di Felice V;Zummo G
通讯作者：
Zummo G
影响因子：
6
作者：
Koti, RS;Yang, WX;Seifalian, AM
通讯作者：
Seifalian, AM
影响因子：
6.4
作者：
Murakami, Junichi;Li, Tao-Sheng;Hamano, Kimikazu
通讯作者：
Hamano, Kimikazu