Sphingopyxis sp. Strain OPL5, an Isoprene-Degrading Bacterium from the Sphingomonadaceae Family Isolated from Oil Palm Leaves.
Sphingopyxis sp. Strain OPL5, an Isoprene-Degrading Bacterium from the Sphingomonadaceae Family Isolated from Oil Palm Leaves.
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DOI：
10.3390/microorganisms8101557
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发表时间：
2020-10-10
期刊：
影响因子：
4.5
通讯作者：
Murrell JC
中科院分区：
文献类型：
--
作者：
Larke-Mejía NL;Carrión O;Crombie AT;McGenity TJ;Murrell JC
易挥发的次级代谢物异戊二烯由树木大量释放到大气中，对空气质量和气候产生重要影响。油棕榈树是异戊二烯排放量最高的树种之一，在亚洲大片地区日益占据农林业的主导地位，其对气候的影响也存在相关的不确定性。能够利用异戊二烯作为碳源生长的微生物已经在土壤和树木的叶圈中被鉴定，并且大多数是放线菌的成员。在这里，我们使用DNA稳定同位素探测来鉴定与油棕榈叶相关并栖息在周围土壤中的异戊二烯降解细菌。其中最丰富的异戊二烯降解剂的叶相关的社区的成员的Sphingomonadales，虽然没有代表这个顺序是以前已知的降解异戊二烯。根据这些数据，我们获得了富集中最丰富的异戊二烯降解剂的代表，包括鞘氨醇单胞菌属菌株OPL 5（鞘氨醇单胞菌目），其能够以异戊二烯作为唯一的碳和能量来源生长。菌株OPL5的基因组测序，以及一种新的戈登氏菌菌株，证实了它们的异戊二烯降解途径，并拓宽了我们对这一重要细菌性状的遗传和分类多样性的认识。
The volatile secondary metabolite, isoprene, is released by trees to the atmosphere in enormous quantities, where it has important effects on air quality and climate. Oil palm trees, one of the highest isoprene emitters, are increasingly dominating agroforestry over large areas of Asia, with associated uncertainties over their effects on climate. Microbes capable of using isoprene as a source of carbon for growth have been identified in soils and in the tree phyllosphere, and most are members of the Actinobacteria. Here, we used DNA stable isotope probing to identify the isoprene-degrading bacteria associated with oil palm leaves and inhabiting the surrounding soil. Among the most abundant isoprene degraders of the leaf-associated community were members of the Sphingomonadales, although no representatives of this order were previously known to degrade isoprene. Informed by these data, we obtained representatives of the most abundant isoprene degraders in enrichments, including Sphingopyxis strain OPL5 (Sphingomonadales), able to grow on isoprene as the sole source of carbon and energy. Sequencing of the genome of strain OPL5, as well as a novel Gordonia strain, confirmed their pathways of isoprene degradation and broadened our knowledge of the genetic and taxonomic diversity of this important bacterial trait.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
DOI：
10.1073/pnas.1812668115
发表时间：
2018-12-18
影响因子：
11.1
作者：
Crombie AT;Larke-Mejia NL;Emery H;Dawson R;Pratscher J;Murphy GP;McGenity TJ;Murrell JC
通讯作者：
Murrell JC
影响因子：
5.1
作者：
El Khawand, Myriam;Crombie, Andrew T.;Murrell, J. Colin
通讯作者：
Murrell, J. Colin
影响因子：
4.5
作者：
Dawson, Robin A.;Larke-Mejia, Nasmille L.;Murrell, J. Colin
通讯作者：
Murrell, J. Colin
影响因子：
6.9
作者：
Hedin, G.;Rynback, J.;Lore, B.
通讯作者：
Lore, B.