A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans.
A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans.
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一种新的习惯化团体训练程序表明，突触前谷氨酸释放有助于秀丽隐杆线虫的长期记忆。
DOI：
10.1101/lm.46802
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发表时间：
2002
影响因子：
2
通讯作者：
C. Rankin
中科院分区：
文献类型：
--
作者：
Jacqueline K. Rose;K. Kaun;C. Rankin
在这里报道的实验中，我们已经开发了一种新的组训练协议，用于评估秀丽隐杆线虫习惯化的长期记忆。我们使用新的协议复制了原始单蠕虫协议的所有主要发现：（1）分布式训练产生了长期的训练保留，大规模训练没有;（2）长刺激间隔（ISI）的分布式训练产生了长期的保留，短ISI没有;分布式训练的长时记忆是蛋白质合成依赖性的，热休克可阻断长时记忆。此外，我们已经证明，习惯化的长期记忆是分级的，这取决于训练中刺激块的数量。阻滞间隔必须>40分钟，以实现长期的训练保持。最后，我们测试了一种蠕虫的习惯化训练的长期记忆，这种蠕虫的感觉神经元中的囊泡谷氨酸转运体发生了突变（eat-4）。这些Eat-4蠕虫的结果表明，感觉神经元释放的谷氨酸在习惯化的长期记忆的形成中起着重要作用。
In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be >40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.
秀丽隐杆线虫的摄食遗传学。
影响因子：
3.3
作者：
Avery,L
通讯作者：
Avery,L