Chronological protein synthesis in regenerating rat liver
Chronological protein synthesis in regenerating rat liver
复制标题
再生大鼠肝脏中蛋白质合成的时间顺序
DOI：
10.1002/elps.201500019
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发表时间：
2015-05
期刊：
影响因子：
2.9
通讯作者：
He, Dacheng
中科院分区：
文献类型：
--
作者：
Guo, Fuzheng;Huang, Lingyun;Xiao, Xueyuan;He, Dacheng
肝脏再生已被研究了数十年；然而，其调控机制仍不明确。在本研究中，我们运用一种新的蛋白质组学技术——35S体内标记动态分析，对大鼠再生肝脏中的蛋白质合成进行了动态追踪。
Liver regeneration has been studied for decades; however, its regulation remains unclear. In this study, we report a dynamic tracing of protein synthesis in rat regenerating liver with a new proteomic technique, 35S in vivo labeling analysis for dynamic proteomics (SiLAD). Conventional proteomic techniques typically measure protein alteration in accumulated amounts. The SiLAD technique specifically detects protein synthesis velocity instead of accumulated amounts of protein through 35S pulse labeling of newly synthesized proteins, providing a direct way for analyzing protein synthesis variations. Consequently, protein synthesis within short as 30 min was visualized and protein regulations in the first 8 h of regenerating liver were dynamically traced. Further, the 3.5–5 h post partial hepatectomy (PHx) was shown to be an important regulatory turning point by acute regulation of many proteins in the initiation of liver regeneration.
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DOI：
10.1002/hep.22588
发表时间：
2008-11-01
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Liedtke, Christian;Trautwein, Christian
通讯作者：
Trautwein, Christian
影响因子：
8
作者：
J. Sobczak;N. Mechti;M. Tournier;J. Blanchard;M. Duguet
通讯作者：
J. Sobczak;N. Mechti;M. Tournier;J. Blanchard;M. Duguet
影响因子：
7.4
作者：
Hoopmann, Michael P.;Chavez, Juan D.;Bruce, James E.
通讯作者：
Bruce, James E.
影响因子：
13.5
作者：
Fausto, N;Campbell, JS;Riehle, KJ
通讯作者：
Riehle, KJ
影响因子：
4.8
作者：
Bergin, David A.;Greene, Catherine M.;McElvaney, Noel G.
通讯作者：
McElvaney, Noel G.