The Distinct Roles of T Cell-Derived Cytokines and a Novel Follicular Dendritic Cell-Signaling Molecule 8D6 in Germinal Center-B Cell Differentiation
The Distinct Roles of T Cell-Derived Cytokines and a Novel Follicular Dendritic Cell-Signaling Molecule 8D6 in Germinal Center-B Cell Differentiation
复制标题
T 细胞衍生的细胞因子和新型滤泡树突状细胞信号分子 8D6 在生发中心 B 细胞分化中的独特作用
作者：
Xin Zhang;Li Li;Jaeho Jung;S. Xiang;C. Hollmann;Y. Choi
生发中心 B (GC-B) 细胞通过与 T 细胞和滤泡树突细胞 (FDC) 相互作用分化为记忆 B 细胞和浆细胞 (PC)。活化的 T 细胞和 FDC 在此过程中发挥着不同的作用。详细的动力学实验表明，活化的T细胞分泌的细胞因子决定了GC-B细胞分化的途径。 IL-4 引导 GC-B 细胞分化为记忆 B 细胞，而 IL-10 引导它们分化为 PC。 FDC/HK 细胞不指导任一途径，但为 GC-B 细胞的增殖提供信号。 FDC 产生的新型 FDC 信号分子 8D6 (FDC-SM-8D6) 可增强 GC 中 PC 的生成。 FDC-SM-8D6 特异性 mAb 可阻断 PC 生成和 IgG 分泌，但不会阻断记忆 B 细胞增殖。表达 FDC-SM-8D6 的 COS 细胞增强 GC-B 细胞增殖和 Ab 分泌，但 mAb 8D6 可以阻断这一作用。在具有 B 细胞亚群的培养物中，在具有 CD27+ B 细胞的培养物中，PC 生成受到 mAb 8D6 的抑制，但在具有 CD27− B 细胞的培养物中则不然，这表明 CD27+ PC 前体是 FDC-SM-8D6 刺激的特定靶标。
Germinal center-B (GC-B) cells differentiate into memory B cells and plasma cells (PC) through interaction with T cells and follicular dendritic cells (FDC). Activated T cell and FDC play distinct roles in this process. The detailed kinetic experiments revealed that cytokines secreted by activated T cells determined the pathway of GC-B cell differentiation. IL-4 directs GC-B cells to differentiate into memory B cells, whereas IL-10 steers them into PC. FDC/HK cells do not direct either pathway, but provide signals for proliferation of GC-B cells. A novel FDC-signaling molecule 8D6 (FDC-SM-8D6) produced by FDC augments PC generation in the GC. FDC-SM-8D6-specific mAb blocked PC generation and IgG secretion but not memory B cell proliferation. COS cells expressing FDC-SM-8D6 enhanced GC-B cell proliferation and Ab secretion, which was blocked by mAb 8D6. In the cultures with B cell subsets, PC generation was inhibited by mAb 8D6 in the cultures with CD27+ B cells but not in the culture with CD27− B cells, suggesting that CD27+ PC precursor is the specific target of FDC-SM-8D6 stimulation.
影响因子：
4.4
作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
通讯作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
用新型单克隆抗体 HJ2 纯化的人滤泡树突状细胞的特性。
影响因子：
4.3
作者：
Butch,AW;Hug,BA;Nahm,MH
通讯作者：
Nahm,MH