Mouse tissues express multiple splice variants of prominin-1.
Mouse tissues express multiple splice variants of prominin-1.
复制标题
DOI：
10.1371/journal.pone.0012325
复制
发表时间：
2010-08-20
期刊：
影响因子：
3.7
通讯作者：
Medema JP
中科院分区：
文献类型：
--
作者：
Kemper K;Tol MJ;Medema JP
一种高度糖基化的五aspan膜蛋白，主要以其作为（癌症）干细胞标记物的功能而闻名，尽管它也可以在分化细胞中检测到。小鼠的突起蛋白1的表达受到8种不同变体剪接的严重调控。迄今为止，人们对pronin -1及其剪接变体（sv）的功能或表达模式尚不清楚。在本研究中，我们分析了几种小鼠组织中突起-1剪接变异体在mRNA水平上的表达，发现大多数SVs在组织中广泛表达，但一组特定的SVs表达谱受限制得多。例如，睾丸只表达SV3和SV7。此外，SV8仅在眼睛中检测到。有趣的是，敲除突出蛋白1的小鼠并没有出现明显的异常，但确实表现出失明的迹象，这表明SV8在该组织中具有特定的功能。此外，在数据库中搜索小鼠prominent -1基因中假定的启动子区域，发现了三个可能与特定sv相关的潜在启动子区域。有趣的是，对于SV7和SV8，可以确定一个特定的潜在启动子区域。综上所述，大多数小鼠prominent -1剪接变异体在小鼠组织中广泛表达。然而，一些变体的特异性表达，可能是由特定的启动子驱动的，表明这些变体在某些组织中具有不同的调控和潜在的重要功能。
Prominin-1, a heavily glycosylated pentaspan membrane protein, is mainly known for its function as a marker for (cancer) stem cells, although it can also be detected on differentiated cells. Mouse prominin-1 expression is heavily regulated by splicing in eight different variants. The function or the expression pattern of prominin-1 and its splice variants (SVs) is thus far unknown. In this study, we analyzed the expression of the prominin-1 splice variants on mRNA level in several mouse tissues and found a broad tissue expression of the majority of SVs, but a specific set of SVs had a much more restricted expression profile. For instance, the testis expressed only SV3 and SV7. Moreover, SV8 was solely detected in the eye. Intriguingly, prominin-1 knockout mice do not suffer from gross abnormalities, but do show signs of blindness, which suggest that SV8 has a specific function in this tissue. In addition, databases searches for putative promoter regions in the mouse prominin-1 gene revealed three potential promoter regions that could be linked to specific SVs. Interestingly, for both SV7 and SV8, a specific potential promoter region could be identified. To conclude, the majority of mouse prominin-1 splice variants are widely expressed in mouse tissues. However, specific expression of a few variants, likely driven by specific promoters, suggests distinct regulation and a potential important function for these variants in certain tissues.
登录
查看更多内容
影响因子：
23.9
作者：
Todaro, Matilde;Alea, Mileidys Perez;Stassi, Giorgio
通讯作者：
Stassi, Giorgio
影响因子：
8.4
作者：
Monzani, Elena;Facchetti, Floriana;La Porta, Caterina A. M.
通讯作者：
La Porta, Caterina A. M.
影响因子：
64.8
作者：
O'Brien, Catherine A.;Pollett, Aaron;Dick, John E.
通讯作者：
Dick, John E.
影响因子：
3.6
作者：
Wilkerson DC;Murphy LA;Sarge KD
通讯作者：
Sarge KD
影响因子：
15.9
作者：
Shmelkov, Sergey V.;Butler, Jason M.;Rafii, Shahin
通讯作者：
Rafii, Shahin