Interaction of HSF1 and HSF2 with the Hspa1b promoter in mouse epididymal spermatozoa.
Interaction of HSF1 and HSF2 with the Hspa1b promoter in mouse epididymal spermatozoa.
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DOI：
10.1095/biolreprod.107.066241
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发表时间：
2008-08
影响因子：
3.6
通讯作者：
Sarge KD
中科院分区：
文献类型：
--
作者：
Wilkerson DC;Murphy LA;Sarge KD
Hspa1b基因是受精后最早表达的基因之一，早在胚胎发生的单细胞阶段就在雄性原核中表达。这种表达可以在没有压力的情况下发生，并在次要的合子基因组激活期间启动。当HSPA1B水平耗尽时，胚胎发育到胚细胞阶段的显著减少，支持该蛋白对胚胎生存能力的重要性。然而，Hspa1b在小ZGA中表达的机制尚不清楚。在本报告中，我们研究了HSF1和HSF2在精子发生后期标记Hspa1b中的作用。Western blot结果显示，HSF1和HSF2均存在于附睾精子中，免疫荧光分析显示，这些细胞中的一些HSF1和HSF2蛋白在dapi染色的DNA区域重叠。染色质免疫沉淀实验结果显示，HSF1、HSF2和Sp1在附睾精子中与Hspa1b启动子结合。此外，我们观察到晚期精子中HSF2与Hspa1b启动子的结合比早期精子中有所增加，这表明在精子发生过程中可能会发生转录因子结合。这些结果支持一个模型，即HSF1、HSF2和Sp1与Hspa1b启动子的结合将允许在小ZGA期间快速形成转录能力状态，从而允许Hspa1b表达。
The Hspa1b gene is one of the first genes expressed after fertilization, with expression observed in the male pronucleus as early as the one-cell stage of embryogenesis. This expression can occur in the absence of stress and is initiated during the minor zygotic genome activation. There is a significant reduction in embryos developing to the blastocyte stage when HSPA1B levels are depleted, supporting the importance of this protein for embryonic viability. However, the mechanism responsible for allowing expression of Hspa1b during the minor ZGA is unknown. In this report, we investigated the role of HSF1 and HSF2 in bookmarking Hspa1b during late spermatogenesis. Western blots show that both HSF1 and HSF2 are present in epididymal spermatozoa and immunofluorescence analysis revealed that some of the HSF1 and HSF2 proteins in these cells overlap the DAPI-stained DNA region. Results from chromatin immunoprecipitation assays showed that HSF1, HSF2, and Sp1 are bound to the Hspa1b promoter in epididymal spermatozoa. Furthermore, we observed an increase in HSF2 binding to the Hspa1b promoter in late spermatids versus early spermatids suggesting a likely period during spermatogenesis when transcription factor binding could occur. These results support a model in which the binding of HSF1, HSF2, and Sp1 to the promoter of Hspa1b would allow the rapid formation of a transcription-competent state during the minor ZGA, thereby allowing Hspa1b expression.
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影响因子：
4.8
作者：
Goodson, ML;Hong, Y;Sarge, KD
通讯作者：
Sarge, KD
影响因子：
64.8
作者：
Christians, E;Davis, AA;Benjamin, IJ
通讯作者：
Benjamin, IJ
影响因子：
11.4
作者：
MAJUMDER, S;MIRANDA, M;DEPAMPHILIS, ML
通讯作者：
DEPAMPHILIS, ML
DOI：
10.1196/annals.1391.005
发表时间：
2007-01-01
期刊：
STRESS RESPONSES IN BIOLOGY AND MEDICINE
影响因子：
--
作者：
Akerfelt, Malin;Trouillet, Diane;Sistonen, Lea
通讯作者：
Sistonen, Lea
影响因子：
11.4
作者：
Kallio, M;Chang, YH;Mezger, V
通讯作者：
Mezger, V