Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis).
Transcriptome sequencing and annotation for the Jamaican fruit bat (Artibeus jamaicensis).
复制标题
牙买加水果蝙蝠（Artibeus Jamaicensis）的转录组测序和注释。
DOI：
10.1371/journal.pone.0048472
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Schountz T
中科院分区：
文献类型：
--
作者：
Shaw TI;Srivastava A;Chou WC;Liu L;Hawkinson A;Glenn TC;Adams R;Schountz T
牙买加果蝠是热带美洲最常见的蝙蝠之一。它被认为是塔卡里贝病毒的潜在储存宿主，塔卡里贝病毒是一种与南美出血热病毒密切相关的阿拉伯病毒。我们使用454和Illumina平台对肺、肾和脾组织进行了转录组测序和注释，以发展该物种作为动物模型。超过10万个重叠群被组装在一起，其中25,000个基因进行了功能注释。在剩下的未注释的重叠群中，80%是在蝙蝠基因组或转录本中发现的。注释基因通过ncRNAs参与从细胞新陈代谢到基因组调控的广泛活动。相互的BLAST最佳命中产生了8785个与老鼠、牛、马和人同源的序列。来自2378个座位的序列的物种树分析被用来实现95%的引导支持，将蝙蝠作为包含马、狗和牛的分支的姐妹放置。通过对蝙蝠与人之间的替换率估计，鉴定出32个基因，为正选择提供了证据。我们还鉴定了466个免疫相关基因，为研究塔卡贝病毒在该物种中的感染奠定了基础。牙买加果蝠转录组数据集是一种资源，应该为研究蝙蝠的进化和生态学提供额外的候选标记，并为分析寄主反应和疾病病理提供工具。
The Jamaican fruit bat (Artibeus jamaicensis) is one of the most common bats in the tropical Americas. It is thought to be a potential reservoir host of Tacaribe virus, an arenavirus closely related to the South American hemorrhagic fever viruses. We performed transcriptome sequencing and annotation from lung, kidney and spleen tissues using 454 and Illumina platforms to develop this species as an animal model. More than 100,000 contigs were assembled, with 25,000 genes that were functionally annotated. Of the remaining unannotated contigs, 80% were found within bat genomes or transcriptomes. Annotated genes are involved in a broad range of activities ranging from cellular metabolism to genome regulation through ncRNAs. Reciprocal BLAST best hits yielded 8,785 sequences that are orthologous to mouse, rat, cattle, horse and human. Species tree analysis of sequences from 2,378 loci was used to achieve 95% bootstrap support for the placement of bat as sister to the clade containing horse, dog, and cattle. Through substitution rate estimation between bat and human, 32 genes were identified with evidence for positive selection. We also identified 466 immune-related genes, which may be useful for studying Tacaribe virus infection of this species. The Jamaican fruit bat transcriptome dataset is a resource that should provide additional candidate markers for studying bat evolution and ecology, and tools for analysis of the host response and pathology of disease.
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影响因子：
4.3
作者：
Altenhoff, Adrian M.;Dessimoz, Christophe
通讯作者：
Dessimoz, Christophe
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
DOI：
10.4269/ajtmh.1982.31.1260
发表时间：
1982-01-01
影响因子：
3.3
作者：
CALISHER, CH;KINNEY, RM;FRANCY, DB
通讯作者：
FRANCY, DB
影响因子：
64.8
作者：
Bustamante, CD;Fledel-Alon, A;Clark, AG
通讯作者：
Clark, AG
DOI：
10.4269/ajtmh.1963.12.640
发表时间：
1963-01-01
影响因子：
3.3
作者：
DOWNS, WG;GREENHALGH, AH;AITKEN, THG
通讯作者：
AITKEN, THG