Protocol for purification of cells in their native state using reversible aptamer-antidote pairs.
Protocol for purification of cells in their native state using reversible aptamer-antidote pairs.
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DOI：
10.1016/j.xpro.2023.102348
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发表时间：
2023-09-15
期刊：
影响因子：
--
通讯作者：
Sullenger, Bruce A.
中科院分区：
文献类型：
--
作者：
Requena, Martin D.;Gray, Bethany Powell;Sullenger, Bruce A.
从复杂混合物中分离细胞是许多临床和研究应用中的关键步骤，但标准分离方法可能会影响细胞的生物学特性，并且难以逆转。在这里，我们提出了一种方法来分离和恢复细胞到其天然状态，使用适配体结合表皮生长因子受体（EGFR+）细胞和互补的反义寡核苷酸反向结合。有关本方案使用和执行的完整详细信息，请参见Gray等人。特定细胞类型和活细胞的高纯度分离纯化后细胞恢复到其天然状态适用于不同的适体-解毒剂对适用于血液、匀浆组织或其他复杂细胞混合物出版商说明：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指南。从复杂混合物中分离细胞是许多临床和研究应用中的关键步骤，但标准分离方法可能会影响细胞的生物学特性，并且难以逆转。在这里，我们提出了一种方法来分离和恢复细胞到其天然状态，使用适配体结合表皮生长因子受体（EGFR+）细胞和互补的反义寡核苷酸反向结合。
Cell isolation from complex mixtures is a key step in many clinical and research applications, but standard isolation methods may affect the cell’s biology and are difficult to reverse. Here, we present a method to isolate and restore cells to their native state using an aptamer that binds epidermal growth factor receptor (EGFR+)cells and a complementary antisense oligonucleotide to reverse binding. For complete details on the use and execution of this protocol, please refer to Gray et al. High-purity isolation of specific cell types and viable cells Cells are returned to their native state post-purification Adaptable for use with different aptamer-antidote pairs Adaptable for use with blood, homogenized tissue, or other complex cell mixtures Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Cell isolation from complex mixtures is a key step in many clinical and research applications, but standard isolation methods may affect the cell’s biology and are difficult to reverse. Here, we present a method to isolate and restore cells to their native state using an aptamer that binds epidermal growth factor receptor (EGFR+) cells and a complementary antisense oligonucleotide to reverse binding.
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影响因子：
46.9
作者：
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Sullenger BA
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影响因子：
64.8
作者：
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通讯作者：
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