Monocyte-macrophage-derived acidic isoferritins: Normal feedback regulators of granulocyte-macrophage progenitor cells in vitro
Monocyte-macrophage-derived acidic isoferritins: Normal feedback regulators of granulocyte-macrophage progenitor cells in vitro
复制标题
单核细胞-巨噬细胞衍生的酸性异铁蛋白：体外粒细胞-巨噬细胞祖细胞的正常反馈调节因子
DOI：
--
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
H. Castro
中科院分区：
文献类型：
--
作者：
H. Broxmeyer;J. Bognacki;P. Ralph;M. Dorner;Li Lu;H. Castro
最近发现一种白血病相关的抑制粒细胞-巨噬细胞祖细胞（CFU-GM）的活性（LIA）作为酸性异铁蛋白，现已在正常骨髓和血细胞中检测到这种活性。这种活性的检测依赖于粒细胞-巨噬细胞集落刺激因子（GM-CSF）对CFU-GM的刺激，而一些GM-CSF的条件培养基（CM）来源（人胎盘和单核细胞、小鼠巨噬细胞和WEHI-3）含有低水平的酸性异铁蛋白，可降低集落的形成。这种活性的失活或去除增加了CM的刺激能力。缺乏酸性异铁蛋白或原本缺乏这种活性的CM（人GCT、5637、Mo、淋巴细胞、小鼠L细胞或豚草丝裂原刺激的脾细胞）增加了检测酸性异铁蛋白抑制活性的灵敏度。这种活性选择性地从正常单核细胞和巨噬细胞中被抑制和释放。这种活性仅限于单核吞噬细胞，因为只有单核和巨噬细胞的连续细胞系或能够诱导这一谱系的细胞系含有并释放酸性异铁蛋白抑制活性。起源细胞和作用的靶细胞表明，酸性异铁蛋白抑制活性可以被认为是一种负反馈调节器，至少在体外是这样。
The recent identification of a leukemia-associated inhibitory activity (LIA) against granulocyte-macrophage progenitor cells (CFU-GM) as acidic isoferritins has now led to detection of this activity in normal bone marrow and blood cells. Detection of this activity depends on stimulation of CFU-GM by granulocyte-macrophage colony stimulatory factors (GM-CSF), and some conditioned media (CM) sources of GM-CSF (human placental and monocyte, mouse macrophage and WEHI-3) contained low levels of acidic isoferritin that lowered colony formation. Inactivation or removal of this activity increased the stimulatory capacity of the CM. CM depleted of acidic isoferritins or CM originally devoid of this activity (human GCT, 5637, Mo, lymphocytes: mouse L cells or pokeweed-mitogen-stimulated spleen cells) increased the sensitivity of the assay to detect acidic isoferritin inhibitory activity. This activity was selectively contained and released from normal monocytes and macrophages. Restriction of this activity to mononuclear phagocytes was substantiated, as only continuous cell lines of monocytes and macrophages or lines capable of induction to this lineage contained and released acidic isoferritin inhibitory activity. The cells of origin and target cells of action suggest that acidic isoferritin-inhibitory activity can be considered as a negative feedback regulator, at least in vitro.
影响因子：
20.3
作者：
J. Dipersio;J. Brennan;M. Lichtman;C. Abboud;F. Kirkpatrick
通讯作者：
J. Dipersio;J. Brennan;M. Lichtman;C. Abboud;F. Kirkpatrick
人 T 淋巴细胞衍生的粒细胞巨噬细胞集落刺激因子的纯化和表征。
影响因子：
20.3
作者：
Lusis,AJ;Quon,DH;Golde,DW
通讯作者：
Golde,DW