Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC
Use of a bacterial expression vector to map the varicella-zoster virus major glycoprotein gene, gC
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使用细菌表达载体绘制水痘带状疱疹病毒主要糖蛋白基因 gC 的图谱
DOI：
10.1128/jvi.53.1.81-88.1985
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发表时间：
1985
影响因子：
5.4
通讯作者：
R. Zivin
中科院分区：
文献类型：
--
作者：
R. Ellis;P. Keller;R. Lowe;R. Zivin
水痘-带状疱疹病毒（VZV）基因组编码至少三个主要糖蛋白基因。在病毒基因产物中，gC基因产物是最丰富的糖蛋白，并诱导大量的体液免疫应答（Keller等人，J. Virol. 52：293-297，1984）。我们利用两种独立的方法来定位gC基因。将随机消化的VZV DNA的小片段插入细菌表达载体中。用抗gC的单克隆抗体对由该载体文库转化的细菌菌落进行抗原表达的血清学筛选。gC抗原阳性克隆的质粒DNA的杂交显示与VZV Us片段的3'端同源。此外，从VZV感染的细胞的mRNA杂交选择的一组VZV DNA重组质粒，并在体外翻译，和多肽产物的免疫沉淀的恢复期带状疱疹血清或gC的单克隆抗体。该分析显示，编码可被抗gC抗体沉淀的70，000-道尔顿多肽的mRNA定位于HindIII C片段，其限制整个Us区域。我们的结论是VZV gC糖蛋白基因定位于Us区的3'端，并表达为70，000-道尔顿的初级翻译产物。这些结果与最近报道的我们的DNA序列一致（A. J. Davison，EMBO J. 2：2203-2209，1983）。此外，糖基化似乎不是gC糖蛋白抗原性的主要部分所需的。我们还报告了其他八个VZV初级翻译产物的暂定地图分配。
The genome of varicella-zoster virus (VZV) encodes at least three major glycoprotein genes. Among viral gene products, the gC gene products are the most abundant glycoproteins and induce a substantial humoral immune response (Keller et al., J. Virol. 52:293-297, 1984). We utilized two independent approaches to map the gC gene. Small fragments of randomly digested VZV DNA were inserted into a bacterial expression vector. Bacterial colonies transformed by this vector library were screened serologically for antigen expression with monoclonal antibodies to gC. Hybridization of the plasmid DNA from a gC antigen-positive clone revealed homology to the 3' end of the VZV Us segment. In addition, mRNA from VZV-infected cells was hybrid selected by a set of VZV DNA recombinant plasmids and translated in vitro, and polypeptide products were immunoprecipitated by convalescent zoster serum or by monoclonal antibodies to gC. This analysis revealed that the mRNA encoding a 70,000-dalton polypeptide precipitable by anti-gC antibodies mapped to the HindIII C fragment, which circumscribes the entire Us region. We conclude that the VZV gC glycoprotein gene maps to the 3' end of the Us region and is expressed as a 70,000-dalton primary translational product. These results are consistent with the recently reported DNA sequence of Us (A.J. Davison, EMBO J. 2:2203-2209, 1983). Furthermore, glycosylation appears not to be required for a predominant portion of the antigenicity of gC glycoproteins. We also report the tentative map assignments for eight other VZV primary translational products.
水痘带状疱疹病毒 DNA 以两种异构体存在。
DOI：
10.1073/pnas.79.1.156
发表时间：
1982
影响因子：
11.1
作者：
Ecker,JR;Hyman,RW
通讯作者：
Hyman,RW
开放阅读框表达载体：使用与 β-半乳糖苷酶的蛋白融合在大肠杆菌中产生抗原的通用方法。
DOI：
10.1073/pnas.80.14.4432
发表时间：
1983
影响因子：
11.1
作者：
Weinstock,GM;apRhys,C;Berman,ML;Hampar,B;Jackson,D;Silhavy,TJ;Weisemann,J;Zweig,M
通讯作者：
Zweig,M
癌症儿童带状疱疹：患病前后血清的放射免疫沉淀谱。
DOI：
10.1093/infdis/147.1.47
发表时间：
1983
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Grose,C
通讯作者：
Grose,C