Wastewater analysis of Mpox virus in a city with low prevalence of Mpox disease: an environmental surveillance study.
Wastewater analysis of Mpox virus in a city with low prevalence of Mpox disease: an environmental surveillance study.
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DOI：
10.1016/j.lana.2023.100639
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发表时间：
2023-12
期刊：
影响因子：
--
通讯作者：
Wu, Fuqing
中科院分区：
文献类型：
--
作者：
Oghuan, Jeremiah;Chavarria, Carlos;Vanderwal, Scout R.;Gitter, Anna;Ojaruega, Akpevwe Amanda;Monserrat, Carlos;Bauer, Cici X.;Brown, Eric L.;Cregeen, Sara Javornik;Deegan, Jennifer;Hanson, Blake M.;Tisza, Michael;Ocaranza, Hector I.;Balliew, John;Maresso, Anthony W.;Rios, Janelle;Boerwinkle, Eric;Mena, Kristina D.;Wu, Fuqing
由于无症状感染和大规模监测的后勤复杂性，在社区一级追踪传染病具有挑战性。废水监测已成为监测包括SARS-CoV-2和Mpox病毒在内的传染病病原体的一个有价值的工具。然而，由于其在社区中的流行率相对较低，因此在废水中检测Mpox病毒特别具有挑战性。在这项研究中，我们的目标是在2023年2月和3月期间，对三种用于检测和跟踪德克萨斯州埃尔帕索废水中Mpox病毒的分子检测方法进行表征。在这项研究中，一个组合的方法，利用三个实时PCR检测靶向C22 L，F3 L，和F8 L基因和测序，以检测和跟踪废水样品中的Mpox病毒。这些样本是在2023年2月和3月期间从德克萨斯州埃尔帕索市的四个下水道收集的。将废水数据与该市报告的临床病例数据进行比较。在所有四个污水处理厂的废水中都检测到了Mpox病毒DNA，而在采样期间只报告了一例Mpox病例。在发现Mpox病例后，在多个污水处理厂仍观察到阳性信号。在沉淀中发现比在废水的上清液中更高的病毒浓度。值得注意的是，在Mpox病例报告前约1-2周观察到病毒浓度呈上升趋势。进一步的测序和流行病学分析为该市未报告的Mpox感染提供了支持证据。我们的分析表明，社区中的天花病例被低估了。研究结果强调了废水监测作为监测传染病的公共卫生工具的价值，即使在低流行地区，也需要提高警惕，以减轻Mpox疾病的传播，以保障全球健康。传染病中心，和。本文的内容完全由作者负责，不一定代表这些资助组织的官方观点。
Tracking infectious diseases at the community level is challenging due to asymptomatic infections and the logistical complexities of mass surveillance. Wastewater surveillance has emerged as a valuable tool for monitoring infectious disease agents including SARS-CoV-2 and Mpox virus. However, detecting the Mpox virus in wastewater is particularly challenging due to its relatively low prevalence in the community. In this study, we aim to characterize three molecular assays for detecting and tracking the Mpox virus in wastewater from El Paso, Texas, during February and March 2023. In this study, a combined approach utilizing three real-time PCR assays targeting the C22L, F3L, and F8L genes and sequencing was employed to detect and track the Mpox virus in wastewater samples. The samples were collected from four sewersheds in the City of El Paso, Texas, during February and March 2023. Wastewater data was compared with reported clinical case data in the city. Mpox virus DNA was detected in wastewater from all the four sewersheds, whereas only one Mpox case was reported during the sampling period. Positive signals were still observed in multiple sewersheds after the Mpox case was identified. Higher viral concentrations were found in the pellet than in the supernatant of wastewater. Notably, an increasing trend in viral concentration was observed approximately 1–2 weeks before the reporting of the Mpox case. Further sequencing and epidemiological analysis provided supporting evidence for unreported Mpox infections in the city. Our analysis suggests that the Mpox cases in the community is underestimated. The findings emphasize the value of wastewater surveillance as a public health tool for monitoring infectious diseases even in low-prevalence areas, and the need for heightened vigilance to mitigate the spread of Mpox disease for safeguarding global health. Center of Infectious Diseases at , the , and the . The content of this paper is solely the responsibility of the authors and does not necessarily represent the official views of these funding organizations.
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影响因子：
9.8
作者：
de Jonge, Eline F.;Koelewijn, Jaap M.;van der Drift, Anne-Merel R.;Beek, Rudolf F. H. J. van der;Nagelkerke, Erwin;Lodder, Willemijn J.
通讯作者：
Lodder, Willemijn J.
影响因子：
82.9
作者：
Isidro, Joana;Borges, Vitor;Pinto, Miguel;Sobral, Daniel;Santos, Joao Dourado;Nunes, Alexandra;Mixao, Veronica;Ferreira, Rita;Santos, Daniela;Duarte, Silvia;Vieira, Luis;Borrego, Maria Jose;Nuncio, Sofia;de Carvalho, Isabel Lopes;Pelerito, Ana;Cordeiro, Rita;Gomes, Joao Paulo
通讯作者：
Gomes, Joao Paulo
影响因子：
56.3
作者：
Lapa, Daniele;Carletti, Fabrizio;Mazzotta, Valentina;Matusali, Giulia;Pinnetti, Carmela;Meschi, Silvia;Gagliardini, Roberta;Colavita, Francesca;Mondi, Annalisa;Minosse, Claudia;Scorzolini, Laura;Cicalini, Stefania;Maffongelli, Gaetano;Specchiarello, Eliana;Camici, Marta;Bettini, Aurora;Baldini, Francesco;Francalancia, Massimo;Mizzoni, Klizia;Garbuglia, Anna Rosa;Nicastri, Emanuele;Girardi, Enrico;Antinori, Andrea;Vaia, Francesco;Maggi, Fabrizio
通讯作者：
Maggi, Fabrizio
DOI：
10.1080/09603123.2022.2159017
发表时间：
2023-01-05
影响因子：
3.2
作者：
Gitter, Anna;Bauer, Cici;Mena, Kristina D.
通讯作者：
Mena, Kristina D.
DOI：
10.1073/pnas.1808798115
发表时间：
2018-11-06
影响因子：
11.1
作者：
Brouwer, Andrew F.;Eisenberg, Joseph N. S.;Eisenberg, Marisa C.
通讯作者：
Eisenberg, Marisa C.