Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes
Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes
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显示肌纤维母细胞和组织细胞表型的大鼠纤维肉瘤来源的可移植肿瘤系（SS）和培养细胞系（SS-P 和 SS-A3-1）的特征
作者：
J. Yamate;M. Iwaki;D. Kumagai;Y. Tsukamoto;M. Kuwamura;S. Nakatsuji;I. Tsunenari;T. Kotani;S. Sakuma
摘要：在同基因大鼠中建立了可移植肿瘤系（SS），该肿瘤源自 24 个月大的雄性 F344 大鼠下颌下唾液腺中出现的自发性纤维肉瘤。从SS诱导细胞系(SS-P)，并从SS-P分离克隆细胞系(SS-A3-1)。原发肿瘤由椭圆形至纺锤形细胞组成，细胞排列成束，胶原纤维丰富；超微结构上，肿瘤细胞呈现梭形形态，具有突出的粗面内质网。 SS肿瘤显示出明显的交错束状和人字形生长模式。 SS-P和SS-A3-1在形态上彼此相似，由椭圆形、梭形或多角形细胞和偶尔的多核巨细胞组成。 SS-P和SS-A3-1诱导的肿瘤在组织学上与SS肿瘤相似。免疫组织化学显示，原发肿瘤、SS肿瘤以及SS-P和SS-A3-1以及SS-P和SS-A3-1培养物诱导的肿瘤中的所有细胞均对波形蛋白呈阳性反应。有趣的是，对 ED1（大鼠巨噬细胞/组织细胞特异性抗体）和 α-平滑肌肌动蛋白 (α-SMA) 发生反应的肿瘤细胞出现在 SS 肿瘤以及 SS-P 和 SS-A3-1 以及 SS-P 和 SS-A3-1 培养物诱导的肿瘤中。还通过电子显微镜观察到具有组织细胞精细结构的细胞和具有细胞质肌动蛋白样微丝的肌纤维母细胞。目前的大鼠纤维肉瘤来源的可移植肿瘤系（SS）和细胞系（SS-P和SS-A3-1）可能表达肌纤维母细胞和组织细胞表型，这可能取决于周围条件。这些细胞系可能有助于研究肿瘤成纤维细胞的表型可塑性机制。
Abstract A transplantable tumour line (SS) was established in syngeneic rats from a spontaneous fibrosarcoma that had arisen in the submandibular salivary gland of a 24-month-old male F344 rat. A cell line (SS-P) was induced from SS, and a cloned cell line (SS-A3-1) was isolated from SS-P. The primary tumour consisted of oval to spindle-shaped cells arranged in bundles with abundant collagen fibres; ultrastructurally, neoplastic cells exhibited fusiform morphology with prominent rough endoplasmic reticulum. SS tumours showed marked interlacing fascicle and herring-bone growth patterns. SS-P and SS-A3-1 were simmilar morphologically to each other, consisting of oval, spindle or polygonal cells and occasional multinucleated giant cells. Tumours induced by SS-P and SS-A3-1 were histologically similar to SS tumours. Immunohistochemically, all cells in the primary tumour, SS tumours and tumours induced both by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures gave a positive reaction to vimentin. Interestingly, neoplastic cells reacting to ED1 (rat macrophage/histiocyte-specific antibody) and α-smooth muscle actin (α-SMA) appeared in SS tumours and tumours induced by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures. Cells with histiocytic fine structures and myofibroblastic cells with cytoplasmic actin-like microfilaments were also observed by electron microscopy. The present rat fibrosarcoma-derived transplantable tumour line (SS) and cell lines (SS-P and SS-A3-1) might express myofibroblastic and histiocytic phenotypes, probably depending on the surrounding conditions. These cell lines may prove useful for studying the mechanisms of phenotypic plasticity in neoplastic fibroblasts.
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