Mitochondrial membrane potential.
Mitochondrial membrane potential.
复制标题
DOI：
10.1016/j.ab.2017.07.009
复制
发表时间：
2018-07-01
影响因子：
2.9
通讯作者：
Zorov DB
中科院分区：
文献类型：
--
作者：
Zorova LD;Popkov VA;Plotnikov EY;Silachev DN;Pevzner IB;Jankauskas SS;Babenko VA;Zorov SD;Balakireva AV;Juhaszova M;Sollott SJ;Zorov DB
质子泵（络合物I、III和IV）产生的线粒体膜电位（ΔΨm）是氧化磷酸化过程中能量储存的重要组成部分。与质子梯度（ΔpH）一起，ΔΨm形成了氢离子的跨膜电位，氢离子被用来制造ATP。细胞中ΔΨm和ATP的水平保持相对稳定，尽管反映正常生理活动的这两种因素可能出现有限的波动。然而，这两个因素的持续变化可能是有害的。ΔΨmvs正常水平的长期下降或上升可能导致不必要的细胞活力丧失，并引起各种病理。在其他因素中，ΔΨm通过选择性消除功能失调的线粒体在线粒体稳态中起关键作用。它也是离子（除了H+）和蛋白质运输的驱动力，这是线粒体功能健康所必需的。我们提出了ΔΨm对于维持细胞健康和活力至关重要的其他潜在机制，并提供了如何准确测量细胞中ΔΨm的建议，并讨论了伪像的潜在来源。
The mitochondrial membrane potential (ΔΨm) generated by proton pumps (Complexes I, III and IV) is an essential component in the process of energy storage during oxidative phosphorylation. Together with the proton gradient (ΔpH), ΔΨm forms the transmembrane potential of hydrogen ions which is harnessed to make ATP. The levels of ΔΨm and ATP in the cell are kept relatively stable although there are limited fluctuations of both these factors that can occur reflecting normal physiological activity. However, sustained changes in both factors may be deleterious. A long-lasting drop or rise of ΔΨmvs normal levels may induce unwanted loss of cell viability and be a cause of various pathologies. Among other factors, ΔΨm plays a key role in mitochondrial homeostasis through selective elimination of dysfunctional mitochondria. It is also a driving force for transport of ions (other than H+) and proteins which are necessary for healthy mitochondrial functioning. We propose additional potential mechanisms for which ΔΨm is essential for maintenance of cellular health and viability and provide recommendations how to accurately measure ΔΨm in a cell and discuss potential sources of artifacts.
登录
查看更多内容
DOI：
10.1007/978-1-59745-365-3_30
发表时间：
2007-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Davidson, Sean M;Yellon, Derek;Duchen, Michael R
通讯作者：
Duchen, Michael R
影响因子：
3.4
作者：
Antonenko, Yuri N.;Nechaeva, Natalya L.;Zorov, Dmitry B.
通讯作者：
Zorov, Dmitry B.
影响因子：
11.4
作者：
Collins, TJ;Berridge, MJ;Bootman, MD
通讯作者：
Bootman, MD
影响因子：
4.7
作者：
Chinopoulos, C;Tretter, L;Adam-Vizi, V
通讯作者：
Adam-Vizi, V
影响因子：
56.9
作者：
Brinkmann, V;Reichard, U;Zychlinsky, A
通讯作者：
Zychlinsky, A