A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization.
A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization.
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DOI：
10.1083/jcb.200202027
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发表时间：
2002-05-27
期刊：
影响因子：
--
通讯作者：
Goldman RD
中科院分区：
文献类型：
--
作者：
Helfand BT;Mikami A;Vallee RB;Goldman RD
我们目前的证据表明，波形蛋白中间丝（IF）在体内的运动与细胞质动力蛋白。免疫荧光显示，在幼仓鼠肾-21细胞中，动力蛋白和动力肌动蛋白的亚基与波形蛋白的所有结构形式相关。这种关系也支持的存在下，许多组件的动力蛋白和dynactin在IF丰富的细胞骨架制剂。动力素的过表达使IF向细胞表面运动，导致IF的核周清除及其向细胞表面的再分布。IF-丰富的细胞骨架制剂从dynamitin过表达细胞含有相对于控制量减少的动力蛋白，肌动蛋白相关蛋白-1，p150 Glued。相比之下，动力蛋白的量在这些制剂中没有改变，表明它参与连接波形蛋白货物与动力蛋白。结果表明，动力蛋白和动力肌动蛋白是所需的正常组织的波形蛋白IF网络在体内。这些结果与以前的研究还表明，微管（MT）减去和加上端定向电机，细胞质动力蛋白，驱动蛋白之间的平衡是必需的III型IF网络在间期细胞的组装和维护。此外，这些马达在很大程度上负责波形蛋白IF和MT之间的长期公认的关系。
We present evidence that vimentin intermediate filament (IF) motility in vivo is associated with cytoplasmic dynein. Immunofluorescence reveals that subunits of dynein and dynactin are associated with all structural forms of vimentin in baby hamster kidney-21 cells. This relationship is also supported by the presence of numerous components of dynein and dynactin in IF-enriched cytoskeletal preparations. Overexpression of dynamitin biases IF motility toward the cell surface, leading to a perinuclear clearance of IFs and their redistribution to the cell surface. IF-enriched cytoskeletal preparations from dynamitin-overexpressing cells contain decreased amounts of dynein, actin-related protein-1, and p150Glued relative to controls. In contrast, the amount of dynamitin is unaltered in these preparations, indicating that it is involved in linking vimentin cargo to dynactin. The results demonstrate that dynein and dynactin are required for the normal organization of vimentin IF networks in vivo. These results together with those of previous studies also suggest that a balance among the microtubule (MT) minus and plus end–directed motors, cytoplasmic dynein, and kinesin are required for the assembly and maintenance of type III IF networks in interphase cells. Furthermore, these motors are to a large extent responsible for the long recognized relationships between vimentin IFs and MTs.
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DOI：
10.1083/jcb.153.3.449
发表时间：
2001-04-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heath CM;Windsor M;Wileman T
通讯作者：
Wileman T
影响因子：
7.8
作者：
Quintyne, N J;Gill, S R;Eckley, D M;Crego, C L;Compton, D A;Schroer, T A
通讯作者：
Schroer, T A
影响因子：
9.2
作者：
Garces, JA;Clark, IB;Vallee, RB
通讯作者：
Vallee, RB
影响因子：
7.8
作者：
Prahlad, V;Yoon, M;Moir, R D;Vale, R D;Goldman, R D
通讯作者：
Goldman, R D
影响因子：
4.8
作者：
Liao, GJ;Gundersen, GG
通讯作者：
Gundersen, GG