Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus.
Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus.
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DOI：
10.1016/j.jcv.2020.104684
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发表时间：
2020-12
期刊：
影响因子：
--
通讯作者：
Tsang DN
中科院分区：
文献类型：
--
作者：
Mak GC;Lau SS;Wong KK;Chow NL;Lau CS;Lam ET;Chan RC;Tsang DN
我们比较了三种商用的SARS-CoV-2病毒快速抗原检测(RAD)试剂盒。这三种RAD试剂盒的灵敏度比RT-PCR低102-105倍。RAD试剂盒检测新冠肺炎患者呼吸道标本的临床敏感性为22.9%~71.4%。了解不同RAD试剂盒的临床特征可以增加阳性结果的可能性。最近，市场上出现了许多用于诊断新冠肺炎患者的快速抗原检测试剂盒。比较三种市售RAD试剂盒的分析敏感性和临床敏感性。以RT-PCR为参照物，用检测限(LOD)确定检测SARS-CoV-2病毒的分析灵敏度。使用从确诊的新冠肺炎患者采集的呼吸道标本进行临床敏感性评估。LOD结果显示，三种RAD试剂盒的灵敏度比RT-PCR低102~105倍。RAD试剂盒检测新冠肺炎标本的临床敏感性为22.9%~71.4%。尽管RAD试剂盒不如RT-PCR敏感，但了解不同RAD试剂盒的临床特征可以指导我们获得合适的标本进行检测。RAD试剂盒出现阳性结果的可能性将更高。
We compared the three commercially available rapid antigen detection (RAD) kits for detection of SARS-CoV-2 virus. The three RAD kits varied from 102–105 fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9 %–71.4 % for detecting respiratory specimens from COVID-19 patients. Understanding the clinical characteristics of different RAD kits can increase the likelihood of positive results. Numerous rapid antigen detection (RAD) kits for diagnosing COVID-19 patients are available in the market recently. To compare analytical sensitivity and clinical sensitivity for the three commercially available RAD kits. Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients. The LOD results showed that the three RAD kits varied from 102–105 fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9 %–71.4 % for detecting specimens from COVID-19 patients. Although RAD kits were less sensitive than RT-PCR, understanding the clinical characteristics of different RAD kits can guide us to obtain suitable specimens for testing. The likelihood of positive results for RAD kits will be higher.
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影响因子：
8.8
作者：
Chan, K. H.;Lam, S. Y.;Peiris, J. S. M.
通讯作者：
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影响因子：
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作者：
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Poliquin, Guillaume
DOI：
10.1007/s10096-020-04031-2
发表时间：
2020-09-09
影响因子：
4.5
作者：
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通讯作者：
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影响因子：
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作者：
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