Three-dimensional printing of clinical scale and personalized calcium phosphate scaffolds for alveolar bone reconstruction.
Three-dimensional printing of clinical scale and personalized calcium phosphate scaffolds for alveolar bone reconstruction.
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DOI：
10.1016/j.dental.2021.12.141
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发表时间：
2022-03
期刊：
影响因子：
5
通讯作者：
Kaigler, Darnell
中科院分区：
文献类型：
--
作者：
Anderson, Margaret;Dubey, Nileshkumar;Bogie, Kath;Cao, Chen;Li, Junying;Lerchbacker, Joseph;Mendonca, Gustavo;Kauffmann, Frederic;Bottino, Marco C.;Kaigler, Darnell
牙槽骨缺损在形态上是高度可变的，随着缺损大小的增加，用现有的治疗方法和生物材料治疗牙槽骨缺损变得更具挑战性。本研究旨在设计一种方案，用于制造定制的临床规模和患者特异性的生物陶瓷支架，用于重建大型牙槽骨缺损。设计并3D打印了两种基于磷酸钙（CaP）的生物陶瓷支架（海藻酸盐/β-TCP和羟基磷灰石/α-TCP，以下分别称为杂交CaP和骨墨水™），并测定了其与肺泡骨髓干细胞的生物相容性和力学性能。在支架优化之后，开发了一个工作流程，使用锥形束计算机断层扫描（CBCT）成像来设计和3D打印用于临床规模骨缺损的缺陷特异性生物陶瓷支架。与不同填充方向的混合CaP相比，Osteoink™支架具有最高的抗压强度。在细胞培养基中，杂交CaP降解导致pH值降低（6.3），对干细胞的毒性降低；然而，OsteoInk™支架在培养中保持了稳定的pH值（7.2），并通过了ISO细胞毒性标准。最后，我们开发了一个临床可行的实验室工作流程，并利用CBCT成像技术评估了使用OsteoInk™设计定制的和缺陷特异性的CaP支架。结果表明，打印支架具有很高的准确性，可以满足其设计的临床缺陷（打印支架与数字设计的形态学偏差为0.27 mm）。由于其复杂的形态和结构的高度变异性，大的牙槽骨缺损在患者之间很难治疗。我们的研究结果表明，骨墨水™是一种生物相容性材料，可用于临床可接受的3D打印，患者特异性支架精确适合用于牙槽骨重建手术。总的来说，包括CBCT成像、3D手术计划和（生物）打印在内的新兴数字技术可以整合在一起，以解决这一未满足的临床挑战。
Alveolar bone defects can be highly variable in their morphology and, as the defect size increases, they become more challenging to treat with currently available therapeutics and biomaterials. This investigation sought to devise a protocol for fabricating customized clinical scale and patient-specific, bioceramic scaffolds for reconstruction of large alveolar bone defects. Two types of calcium phosphate (CaP)-based bioceramic scaffolds (alginate/β-TCP and hydroxyapatite/α-TCP, hereafter referred to as hybrid CaP and Osteoink™, respectively) were designed, 3D printed, and their biocompatibility with alveolar bone marrow stem cells and mechanical properties were determined. Following scaffold optimization, a workflow was developed to use cone beam computed tomographic (CBCT) imaging to design and 3D print, defect-specific bioceramic scaffolds for clinical-scale bone defects. Osteoink™ scaffolds had the highest compressive strength when compared to hybrid CaP with different infill orientation. In cell culture medium, hybrid CaP degradation resulted in decreased pH (6.3) and toxicity to stem cells; however, OsteoInk™ scaffolds maintained a stable pH (7.2) in culture and passed the ISO standard for cytotoxicity. Finally, a clinically feasible laboratory workflow was developed and evaluated using CBCT imaging to engineer customized and defect-specific CaP scaffolds using OsteoInk™. It was determined that printed scaffolds had a high degree of accuracy to fit the respective clinical defects for which they were designed (0.27 mm morphological deviation of printed scaffolds from digital design). From patient to patient, large alveolar bone defects are difficult to treat due to high variability in their complex morphologies and architecture. Our findings shows that Osteoink™ is a biocompatible material for 3D printing of clinically acceptable, patient-specific scaffolds with precision-fit for use in alveolar bone reconstructive procedures. Collectively, emerging digital technologies including CBCT imaging, 3D surgical planning, and (bio)printing can be integrated to address this unmet clinical challenge.
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影响因子：
6.2
作者：
Kaigler, Darnell;Avila-Ortiz, Gustavo;Giannobile, William V.
通讯作者：
Giannobile, William V.
影响因子：
6
作者：
Bhumiratana S;Vunjak-Novakovic G
通讯作者：
Vunjak-Novakovic G
影响因子：
3.1
作者：
Eniwumide, J. O.;Yuan, H.;de Bruijn, J. D.
通讯作者：
de Bruijn, J. D.
DOI：
10.1073/pnas.0400164101
发表时间：
2004-03-02
影响因子：
11.1
作者：
Jakab, K;Neagu, A;Forgacs, G
通讯作者：
Forgacs, G
影响因子：
6.7
作者：
Jepsen, Soren;Schwarz, Frank;Urban, Istvan
通讯作者：
Urban, Istvan