The fat body transcriptomes of the yellow fever mosquito Aedes aegypti, pre- and post- blood meal.
The fat body transcriptomes of the yellow fever mosquito Aedes aegypti, pre- and post- blood meal.
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DOI：
10.1371/journal.pone.0022573
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hansen IA
中科院分区：
文献类型：
--
作者：
Price DP;Nagarajan V;Churbanov A;Houde P;Milligan B;Drake LL;Gustafson JE;Hansen IA
脂肪体是昆虫中间代谢的主要器官，也是血淋巴蛋白的主要来源。作为我们持续努力了解蚊子脂肪体生理学和确定昆虫控制新目标的一部分，我们对吸血前和吸血后埃及伊蚊的脂肪体进行了转录组分析。我们创建了两个脂肪体非标准化 EST 文库，一个来自非血饲蚊子脂肪体 (NBF)，另一个来自吸血后 24 小时的蚊子 (PBM)。对非标准化文库进行 454 焦磷酸测序，从 NBF 样品中获得 204,578 个可用读数，从 PBM 样品中获得 323,474 个可用读数。读数与现有参考 Ae 的比对。用于分析 NBF 和 PBM 样本之间差异表达的埃及伊蚊转录文库分别显示 116,912 和 115,051 个匹配。来自 NBF 样本的读数的从头组装产生了 15,456 个重叠群，而来自 PBM 样本的读数的组装产生了 15,010 个重叠群。在这些重叠群中总共鉴定出了 123 个新转录本。 NBF 脂肪体文库中突出表达的转录本以编码核糖体蛋白的转录本为代表。 PBM 文库中所有读数的百分之三十五点四是由编码卵黄蛋白的转录本代表的。表达最高的是编码组织蛋白酶b、卵黄蛋白原、卵黄生成羧肽酶和卵黄膜蛋白家族成员的转录物。就转录本表达和表达基因的丰度而言，两种脂肪体转录组在转录本表达方面彼此存在显着差异。它们反映了进食前脂肪体从静止状态到进食后卵黄基因表达的生理转变。
The fat body is the main organ of intermediary metabolism in insects and the principal source of hemolymph proteins. As part of our ongoing efforts to understand mosquito fat body physiology and to identify novel targets for insect control, we have conducted a transcriptome analysis of the fat body of Aedes aegypti before and in response to blood feeding. We created two fat body non-normalized EST libraries, one from mosquito fat bodies non-blood fed (NBF) and another from mosquitoes 24 hrs post-blood meal (PBM). 454 pyrosequencing of the non-normalized libraries resulted in 204,578 useable reads from the NBF sample and 323,474 useable reads from the PBM sample. Alignment of reads to the existing reference Ae. aegypti transcript libraries for analysis of differential expression between NBF and PBM samples revealed 116,912 and 115,051 matches, respectively. De novo assembly of the reads from the NBF sample resulted in 15,456 contigs, and assembly of the reads from the PBM sample resulted in 15,010 contigs. Collectively, 123 novel transcripts were identified within these contigs. Prominently expressed transcripts in the NBF fat body library were represented by transcripts encoding ribosomal proteins. Thirty-five point four percent of all reads in the PBM library were represented by transcripts that encode yolk proteins. The most highly expressed were transcripts encoding members of the cathepsin b, vitellogenin, vitellogenic carboxypeptidase, and vitelline membrane protein families. The two fat body transcriptomes were considerably different from each other in terms of transcript expression in terms of abundances of transcripts and genes expressed. They reflect the physiological shift of the pre-feeding fat body from a resting state to vitellogenic gene expression after feeding.
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DOI：
10.1073/pnas.0502815102
发表时间：
2005-09-20
影响因子：
11.1
作者：
Bian, G;Shin, SW;Raikhel, AS
通讯作者：
Raikhel, AS
影响因子：
3.5
作者：
COHEN, LW;COGHLAN, VM;DIHEL, LC
通讯作者：
DIHEL, LC
影响因子：
2.6
作者：
Deitsch, K.W.;Raikhel, A.S.
通讯作者：
Raikhel, A.S.
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL
DOI：
10.1073/pnas.88.23.10821
发表时间：
1991-12-01
影响因子：
11.1
作者：
CHO, WL;DEITSCH, KW;RAIKHEL, AS
通讯作者：
RAIKHEL, AS