Something old, something new, a DNA preparation procedure for long-read genomic sequencing.

Something old, something new, a DNA preparation procedure for long-read genomic sequencing.
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DOI:
10.1139/gen-2021-0029
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发表时间:
2021-07-27
期刊:
影响因子:
3.1
通讯作者:
Xu S
Xu S
中科院分区:
生物学3区
文献类型:
--
作者:
Wang H;Xu S

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提取高分子量DNA对于成功的长读段DNA测序至关重要。在这里,我们提出了一个DNA制备程序相结合的CTAB DNA提取协议和短读消除器试剂盒,它始终产生高产量的高分子量DNA的淡水微甲壳类水蚤。这种方法只需要普通的化学品和设备,为研究人员提供了一个可行的替代DNA提取试剂盒,往往需要专门的工具。
Extracting high molecular weight DNA is critical for successful long read DNA sequencing. Here we present a DNA preparation procedure combining CTAB DNA extraction protocol and a short read eliminator kit, which consistently produces high yields of high molecular weight DNA in the freshwater microcrustacean Daphnia. This method only requires common chemicals and equipment, providing researchers a viable alternative to DNA extraction kits that often require specialized tools.
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