PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.
PtKTI12 genes influence wobble uridine modifications and drought stress tolerance in hybrid poplar.
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PtKTI12 基因影响杂交杨的摆动尿苷修饰和干旱胁迫耐受性。
DOI：
10.1093/treephys/tpaa088
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发表时间：
2020-07
期刊：
影响因子：
4
通讯作者：
Zheng Bo
中科院分区：
文献类型：
--
作者：
Wang Hailang;Xu Chao;Zhang Youbing;Yan Xu;Jin Xiaohuan;Yao Xiaoqing;Chen Peng;Zheng Bo
多亚基延长子复合物通过与RNA聚合酶II和染色质建模相互作用在转录中起关键作用。Kti蛋白已被确定为延长体复合体的辅助蛋白。然而，我们对木本植物中Kti蛋白的了解仍然有限。本研究共鉴定出16个KTI基因同源物。其中，两个KTI12候选者被命名为PtKTI12A和PtKTI12B。尽管PtKTI12A和PtKTI12B在基因表达水平和组织特异性上存在很大差异，但两者都受到高温和干旱胁迫的诱导。PtKTI12A和PtKTI12B RNAi转基因杨树的核苷修饰水平降低，特别是ncm5U（5-氨基甲酰甲基尿嘧啶）和mcm5s2U（5-甲氧基羰基甲基-2-硫尿嘧啶）。同时，在退水条件下，它们的耐旱性有所提高。此外，PtKTI12A和PtKTI12B的蛋白产物具有相似的亚细胞定位和预测三级结构。结果表明，Kti12蛋白参与了杂交杨树tRNA振荡尿苷修饰、胁迫响应和干旱胁迫耐受性。
The multi-subunit Elongator complex plays key roles in transcription by interacting with RNA polymerase II and chromatin modeling. Kti proteins have been identified as the auxiliary protein for the elongator complex. However, our knowledge of Kti proteins in woody plants remains limited. In this study, in total 16 KTI gene homologs were identified in Populus trichocarpa. Among them, the two KTI12 candidates were named PtKTI12A and PtKTI12B. Although PtKTI12A and PtKTI12B were largely different in gene expression level and tissue-specificity, both genes were induced by heat and drought stresses. PtKTI12A and PtKTI12B RNAi transgenic poplar plants showed reduced levels of modified nucleosides, in particular ncm5U (5-carbamoylmethyluridine) and mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine). Meanwhile, their tolerance to drought was improved when subjected to withdrawal of watering. In addition, the protein products of PtKTI12A and PtKTI12B had similar subcellular localisation and predicted tertiary structure. The results suggest that Kti12 proteins are involved in tRNA wobble uridine modification, stress response and drought stress tolerance in hybrid poplar.
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影响因子：
4.9
作者：
Y. Nakai;G. Horiguchi;Kosei Iwabuchi;A. Harada;M. Nakai;I. Hara-Nishimura;T. Yano
通讯作者：
Y. Nakai;G. Horiguchi;Kosei Iwabuchi;A. Harada;M. Nakai;I. Hara-Nishimura;T. Yano
影响因子：
5.3
作者：
Chen P;Jäger G;Zheng B
通讯作者：
Zheng B
DOI：
10.1007/978-3-662-45777-1_4
发表时间：
2015
期刊：
--
影响因子：
--
作者：
S. Nof;José A. Ceroni;W. Jeong;M. Moghaddam
通讯作者：
S. Nof;José A. Ceroni;W. Jeong;M. Moghaddam
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
影响因子：
4.2
作者：
Mehlgarten C;Prochaska H;Hammermeister A;Abdel-Fattah W;Wagner M;Krutyhołowa R;Jun SE;Kim GT;Glatt S;Breunig KD;Stark MJR;Schaffrath R
通讯作者：
Schaffrath R