int-1--a proto-oncogene involved in cell signalling.
int-1--a proto-oncogene involved in cell signalling.
复制标题
int-1——参与细胞信号传导的原癌基因。
作者：
A. P. McMahon;Randall T. Moon
INT-1基因最初被鉴定为一个由小鼠乳腺肿瘤病毒插入而激活的基因。小鼠基因的克隆和测序表明，int-1编码一个41K、370个氨基酸的富含半胱氨酸的蛋白，具有潜在的疏水信号肽序列。表达研究清楚地表明，INT-1进入分泌途径，可能是分泌的，尽管缺乏明确的证据。果蝇INT-1编码无翼基因，Wingless是一种片段极性基因，在每一片段中建立正常模式是必需的。遗传学研究表明，这种无翼蛋白可能是分泌的，因为它是维持邻近细胞稳定的基因表达所必需的。INT-1在青蛙和小鼠发育的早期神经阶段也有表达。在表达良好的小鼠中，int-1RNA被限制在神经管的背侧中线。通过与果蝇的类比，int-1可以在这个结构中指定位置。为了验证这一想法，我们将INT-1 RNA注射到青蛙胚胎中，干扰了正常的INT-1表达。这会导致前神经管的显著和特殊的异常，分叉。因此，在脊椎动物中，int-1似乎能够影响模式事件。
The int-1 gene was originally identified as a locus activated by mouse mammary tumor virus insertion. Cloning and sequencing of the mouse gene indicates that int-1 encodes a 41K, 370 amino acid, cysteine-rich protein with a potential hydrophobic signal peptide sequence. Expression studies clearly indicate that int-1 enters the secretory pathway and is probably secreted, although definitive evidence is lacking. Drosophila int-1 encodes the wingless gene, wingless, a segment-polarity gene, is required for the establishment of normal pattern in each segment. Genetic studies indicate that the wingless protein is probably secreted since it is required for the maintenance of stable gene expression in neighboring cells. int-1 is also expressed during early neural stages of frog and mouse development. In the mouse, where expression is well characterized, int-1 RNA is restricted to the dorsal midline of the neural tube. By analogy with Drosophila, int-1 may operate to specify position within this structure. To test this idea, we have interfered with normal int-1 expression by injection of int-1 RNA into frog embryos. This results in a striking and specific aberration, bifurcation of the anterior neural tube. Thus, it seems possible that in vertebrates int-1 is able to influence patterning events.
影响因子：
2.7
作者：
PERRIMON, N;MAHOWALD, AP
通讯作者：
MAHOWALD, AP