Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group

Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group
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隐藏在树林深处:在马达加斯加变色龙中发现不同的线粒体谱系

DOI:
10.1002/ece3.269
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发表时间:
2012
影响因子:
2.6
通讯作者:
M. Vences
M. Vences
中科院分区:
生物学2区
文献类型:
--
作者:
Gehring;K. A. Tolley;F. S. Eckhardt;T. M. Townsend;T. Ziegler;F. Ratsoavina;F. Glaw ;M. Vences

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我们对马达加斯加的一组变色龙(Chamaeleonidae:Calumma nasutumgroup,包括7个命名种)进行了全面的分子系统发育研究,以考察这种广泛分布的属的遗传和物种多样性。基于215个标本的线粒体基因(ND2)的DNA序列,我们用贝叶斯方法重建了系统发育。我们的结果显示,在形态上很难辨别的几个未命名的线粒体谱系之间存在着严重的分歧。我们使用一些统计的系统发育方法(一般的混合Yule-联合模型;种识别符;净距离)来评估谱系多样性,以客观地界定我们在这里认为是可操作的分类单位(OTU)的谱系,其分类地位在很大程度上尚不清楚。此外,我们详细比较了一个特别不同的支系(C.Boettgericplex)产自马达加斯加北部。为了评估这一组内的物种边界,我们使用了综合分类学方法,结合来自两个独立的分子标记(ND2和CMOS)的证据,以及生殖器和其他外部形态特征,得出结论:一些新发现的OTU是独立的物种(确认的候选物种,CCS),而另一些最好被认为是深层同种谱系(DCL)。我们的分析支持33个OTU,其中7个对应于所描述的种,这表明C。《国家文集》需要修改。
We conducted a comprehensive molecular phylogenetic study for a group of chameleons from Madagascar (Chamaeleonidae:Calumma nasutumgroup, comprising seven nominal species) to examine the genetic and species diversity in this widespread genus. Based on DNA sequences of the mitochondrial gene (ND2) from 215 specimens, we reconstructed the phylogeny using a Bayesian approach. Our results show deep divergences among several unnamed mitochondrial lineages that are difficult to identify morphologically. We evaluated lineage diversification using a number of statistical phylogenetic methods (general mixed Yule‐coalescent model; SpeciesIdentifier; netp‐distances) to objectively delimit lineages that we here consider as operational taxonomic units (OTUs), and for which the taxonomic status remains largely unknown. In addition, we compared molecular and morphological differentiation in detail for one particularly diverse clade (theC. boettgericomplex) from northern Madagascar. To assess the species boundaries within this group we used an integrative taxonomic approach, combining evidence from two independent molecular markers (ND2andCMOS), together with genital and other external morphological characters, and conclude that some of the newly discovered OTUs are separate species (confirmed candidate species, CCS), while others should best be considered as deep conspecific lineages (DCLs). Our analysis supports a total of 33 OTUs, of which seven correspond to described species, suggesting that the taxonomy of theC. nasutumgroup is in need of revision.
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