A highly conserved His-His motif present in alpha1-->3/4fucosyltransferases is required for optimal activity and functions in acceptor binding.
A highly conserved His-His motif present in alpha1-->3/4fucosyltransferases is required for optimal activity and functions in acceptor binding.
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α1→3/4岩藻糖基转移酶中存在高度保守的 His-His 基序，这是受体结合中最佳活性和功能所必需的。
DOI：
10.1093/glycob/cwf075
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发表时间：
2002
期刊：
影响因子：
4.3
通讯作者：
Holmes,EricH
中科院分区：
文献类型：
--
作者：
Sherwood,AnneL;Upchurch,DavidA;Stroud,MarkR;Davis,WilliamC;Holmes,EricH
α1→3/4Fucosyltransferases (FucTs) from several species contain a highly conserved His-His motif adjacent to an enzyme region correlating with the ability to catalyze fucose transfer to type 1 chain acceptors. Site-directed mutagenesis has been employed to analyze structure–function relationships of this His-His motif in human FucT-IV. The results indicate that most changes of His113and His114and nearby residues of FucT-IV reduced the specific activity of the enzymes. Analysis of acceptor properties demonstrated close similarity of most mutants with wild-type FucT-IV, whereas an apparent preference for the H-type II acceptor was observed for the His114mutants. Kinetic studies demonstrated that mutants of His114had a substantially increasedKmfor acceptor compared to other enzymes tested. The dramatic increase in acceptorKmfor the His114mutants, particularly for the nonfucosylated acceptor, suggests that this His-His motif is involved in acceptor binding and perhaps interacts with GlcNAc residues of type 2 acceptors. The presence of fucose in acceptor substrates may promote more efficient substrate binding and presumably partially overcomes the weaker interaction with GlcNAc caused by the mutation.
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影响因子：
4.8
作者：
Costache, M;Apoil, PA;Mollicone, R
通讯作者：
Mollicone, R
影响因子：
2.7
作者：
Kageyama, N;Natsuka, S;Hase, S
通讯作者：
Hase, S
DOI：
--
发表时间：
1994-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. Natsuka;K. M. Gersten;K. Zenita;R. Kannagi;J. Lowe
通讯作者：
S. Natsuka;K. M. Gersten;K. Zenita;R. Kannagi;J. Lowe
影响因子：
4.8
作者：
Oulmouden, A;Wierinckx, A;Julien, R
通讯作者：
Julien, R
绿豆中 GDP-l-Fuc:Asn 连接的 GlcNAc α1,3-岩藻糖基转移酶的纯化、cDNA 克隆和表达*
DOI：
10.1074/jbc.274.31.21830
发表时间：
1999
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
Haralt Leiter;J. Mucha;E. Staudacher;R. Grimm;J. Glössl;F. Altmann
通讯作者：
F. Altmann