Physiological and transcriptome analysis of heteromorphic leaves and hydrophilic roots in response to soil drying in desert Populus euphratica.
Physiological and transcriptome analysis of heteromorphic leaves and hydrophilic roots in response to soil drying in desert Populus euphratica.
复制标题
荒漠胡杨异形叶和亲水根响应土壤干燥的生理和转录组分析
DOI：
10.1038/s41598-017-12091-2
复制
发表时间：
2017-09-22
影响因子：
4.6
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Iqbal A;Wang T;Wu G;Tang W;Zhu C;Wang D;Li Y;Wang H
胡杨被认为是研究高等植物对非生物胁迫反应的树模型，它在以极端干旱胁迫为特征的沙漠生态系统中生存。为了在恶劣的环境条件下生存，植物物种发展出了一些可塑性，例如异形叶子的发育和发达的根系。我们研究了使该物种能够应对干旱引起的严重胁迫的生理和分子机制。异系从线形演化为齿状卵形，在角质层厚度、气孔密度和大小方面表现出显着差异。生理参数、SOD、POD、PPO、CAT活性、游离脯氨酸、可溶性蛋白和MDA含量随土壤干燥而波动。在对照和 4 个湿度梯度方案下监测的根的基因表达谱显示分别上调 124、130、126 和 162，下调 138、251、314、168。木葡聚糖内转葡糖基酶/水解酶基因（XET）在不同湿度梯度下上调，并在烟草中克隆和表达。 XET 启动子序列含有干旱信号响应顺式元件。启动子的表达活性在不同器官中存在差异。转基因烟草植物的过度表达和敲除分析证实了 XET 基因在根系生长和抗旱性中的作用。
Populus euphratica Olivier, which has been considered as a tree model for the study of higher plant response to abiotic stresses, survive in the desert ecosystem characterized by extreme drought stress. To survive in the harsh environmental condition the plant species have developed some plasticity such as the development of heteromorphic leaves and well-developed roots system. We investigated the physiological and molecular mechanisms enabling this species to cope with severe stress caused by drought. The heterophylly, evolved from linear to toothed-ovate shape, showed the significant difference in cuticle thickness, stomata densities, and sizes. Physiological parameters, SOD, POD, PPO, CAT activity, free proline, soluble protein and MDA contents fluctuated in response to soil drying. Gene expression profile of roots monitored at control and 4 moisture gradients regimes showed the up-regulation of 124, 130, 126 and 162 and down-regulation of 138, 251, 314, 168 DEGs, respectively. Xyloglucan endotransglucosylase/ hydrolase gene (XET) up-regulated at different moisture gradients, was cloned and expressed in tobacco. The XET promoter sequence harbors the drought signaling responsive cis-elements. The promoter expression activity varies in different organs. Over-expression and knocked down transgenic tobacco plant analysis confirmed the role of XET gene in roots growth and drought resistance.
登录
查看更多内容
影响因子：
5.3
作者：
Chi M;Bhagwat B;Lane WD;Tang G;Su Y;Sun R;Oomah BD;Wiersma PA;Xiang Y
通讯作者：
Xiang Y
影响因子：
4.1
作者：
FRY, SC;SMITH, RC;MATTHEWS, KJ
通讯作者：
MATTHEWS, KJ
胡杨中 MicroRNA 对植物激素脱落酸反应的全基因组分析
影响因子：
5.6
作者：
Duan H;Lu X;Lian C;An Y;Xia X;Yin W
通讯作者：
Yin W
DOI：
10.1007/978-3-642-02167-1_12
发表时间：
2010-01-01
期刊：
PROGRESS IN BOTANY 71
影响因子：
--
作者：
Hodge, Angela
通讯作者：
Hodge, Angela
影响因子：
2.9
作者：
Banowetz, GM;Dierksen, KP;Stout, R
通讯作者：
Stout, R