FSH-receptor isoforms and FSH-dependent gene transcription in human monocytes and osteoclasts.
FSH-receptor isoforms and FSH-dependent gene transcription in human monocytes and osteoclasts.
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DOI：
10.1016/j.bbrc.2010.02.112
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发表时间：
2010-03-26
影响因子：
3.1
通讯作者：
Blair, Harry C.
中科院分区：
文献类型：
--
作者：
Robinson, Lisa J.;Tourkova, Irina;Wang, Yujuan;Sharrow, Allison C.;Landau, Michael S.;Yaroslavskiy, Beatrice B.;Sun, Li;Zaidi, Mone;Blair, Harry C.
关键词：
单核细胞系列的细胞对促卵泡激素（FSH）的反应机制尚不清楚。我们研究了FSH受体（FSH-R）和FSH的反应，在非转化的人单核细胞和破骨细胞从这些细胞分化。Western blot和PCR证实单核细胞或破骨细胞上FSH-R表达，尽管相对于卵巢对照处于低水平。单核细胞和破骨细胞的FSH-R与卵巢细胞的FSH-R不同，反映了外显子8-10的可变剪接。单核细胞不产生cAMP，这是卵巢细胞中的主要信号，以响应FSH。然而，单核细胞或破骨细胞转录TNFα响应FSH。破骨细胞FSH-R的表达与细胞供体的性别或暴露于性激素没有明显关系。FSH纯度和内毒素污染的对照品均为阴性。在25 ng/ml FSH中2小时后，贴壁CD 14细胞中的未放大cRNA筛选显示RANKL信号蛋白的转录增加。FSH处理后，刺激骨转换的关键蛋白质TNFα和TSG-6的转录增加2-3倍。选定的信号传导、粘附和细胞骨架蛋白的转录物发生了较小但显着的变化。我们的结论是单核细胞和破骨细胞FSH反应偏离卵巢细胞，反映，至少部分，不同的FSH-R亚型。
Cells of the monocyte series respond to follicle stimulating hormone (FSH) by poorly characterized mechanisms. We studied FSH-receptors (FSH-R) and FSH response in nontransformed human monocytes and in osteoclasts differentiated from these cells. Western blot and PCR confirmed FSH-R expression on monocytes or osteoclasts, although at low levels relative to ovarian controls. Monocyte and osteoclast FSH-Rs differed from FSH-R from ovarian cells, reflecting variable splicing in exons 8–10. Monocytes produced no cAMP, the major signal in ovarian cells, in response to FSH. However, monocytes or osteoclasts transcribed TNFα in response to the FSH. No relation of expression of osteoclast FSH-R to the sex of cell donors or to exposure to sex hormones was apparent. Controls for FSH purity and endotoxin contamination were negative. Unamplified cRNA screening in adherent CD14 cells after 2 hours in 25 ng/ml FSH showed increased transcription of RANKL signalling proteins. Transcription of key proteins that stimulate bone turnover, TNFα and TSG-6, increased 2–3 fold after FSH treatment. Smaller but significant changes occurred in transcripts of selected signalling, adhesion, and cytoskeletal proteins. We conclude that monocyte and osteoclast FSH response diverges from that of ovarian cells, reflecting, at least in part, varying FSH-R isoforms.
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DOI：
10.1073/pnas.0606805103
发表时间：
2006-10-03
影响因子：
11.1
作者：
Iqbal, Jameel;Li Sun;Zaidi, Mone
通讯作者：
Zaidi, Mone
影响因子：
3.6
作者：
YEE, JB;HUTSON, JC
通讯作者：
HUTSON, JC
影响因子：
4.1
作者：
Bu, RF;Borysenko, CW;Blair, HC
通讯作者：
Blair, HC
影响因子：
4.8
作者：
Mahoney, David J.;Mikecz, Katalin;Sabokbar, Afsaneh
通讯作者：
Sabokbar, Afsaneh
影响因子：
64.5
作者：
Abe, E;Marians, RC;Zaidi, M
通讯作者：
Zaidi, M