The effect of L-carnitine on oxidative stress responses of experimental contrast-induced nephropathy in rats.
The effect of L-carnitine on oxidative stress responses of experimental contrast-induced nephropathy in rats.
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DOI：
10.1292/jvms.13-0202
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发表时间：
2014-01
期刊：
影响因子：
--
通讯作者：
Onbasili OA
中科院分区：
文献类型：
--
作者：
Boyacioglu M;Turgut H;Akgullu C;Eryilmaz U;Kum C;Onbasili OA
本研究旨在探讨肉碱对造影剂肾病（CIN）大鼠模型的预防作用及其与肾、肝、心、脾和肺组织氧化/抗氧化状态的关系。将28只成年雄性Wistar大鼠分为对照组、造影剂（CM）组、肉碱组和造影剂+肉碱组（CM+肉碱）。将大鼠置于单独的代谢笼中，第2天不饮水24小时。第3天，CM组和CM+肉碱组分别给予对比剂。在第2、3、4天给予左旋肉碱。评估安乐死后右肾的组织病理学改变。测定肾、肝、心、脾和肺组织超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性以及谷胱甘肽（GSH）和丙二醛（MDA）水平。肉碱组大鼠肾（P<0.05）、肝（P<0.001）和脾（P<0.05）组织SOD活性均升高。只有CM组脾脏组织CAT活性降低（P<0.01）。肉碱组肾脏（P<0.05）、肝脏（P<0.001）、脾脏（P<0.001）和肺组织（P<0.01） GSH水平均升高。在肾、肝和肺组织中，CM组MDA水平升高（P<0.001）。组织病理学结果表明，左旋肉碱可能对减轻CIN的负面影响具有预防作用。与此类似，左旋肉碱可能对CIN大鼠模型肾、肝、脾、肺抗氧化状态的稳定起主要作用。
This study was conducted to investigate the prophylactic effects of carnitine against contrast-induced nephropathy (CIN) and its relation to oxidant/antioxidant status in kidney, liver, heart, spleen and lung tissues in a CIN rat model. Twenty-eight adult male Wistar rats were divided into 4 groups, the control, contrast media (CM), carnitine and contrast media+carnitine (CM+carnitine) groups. Animals were placed in individual metabolism cages, and on the 2nd day, rats were deprived of water for 24 hr. On the 3rd day, contrast media were administered to groups CM and CM+carnitine. L-carnitine was administered on days 2, 3 and 4. Histopathological changes were evaluated in the right kidney after euthanization. Superoxide dismutase (SOD) and catalase (CAT) activities and glutathione (GSH) and malondialdehyde (MDA) levels were measured in renal, liver, heart, spleen and lung tissues. The SOD activities in the renal (P<0.05), liver (P<0.001) and spleen (P<0.05) tissues were increased in the carnitine group. The CAT activities in the spleen tissue were decreased (P<0.01) only in the CM group. Renal (P<0.05), liver (P<0.001), spleen (P<0.001) and lung tissue (P<0.01) GSH levels were found to be higher in the carnitine group. In renal, liver and lung tissues, the MDA levels increased in the CM group (P<0.001). The histopathological findings showed that L-carnitine may have a preventative effect in alleviating the negative effects of CIN. Similar to this, L-carnitine may play a major role in the stability of the antioxidant status in the kidney, liver, spleen and lung of the CIN rat model.
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影响因子：
1.3
作者：
Duan, Shao Bin;Wang, Yu Hui;Peng, You Ming
通讯作者：
Peng, You Ming
影响因子：
1.3
作者：
Fishman, E. K.;Reddan, D.
通讯作者：
Reddan, D.
影响因子：
158.5
作者：
Aspelin, P;Aubry, P;Berg, KJ
通讯作者：
Berg, KJ
影响因子：
3.5
作者：
Calò, LA;Pagnin, E;Pessina, AC
通讯作者：
Pessina, AC
影响因子：
6.7
作者：
Hizoh, I;Haller, C
通讯作者：
Haller, C