Molecular Evolution of Far-Red Light-Acclimated Photosystem II.
Molecular Evolution of Far-Red Light-Acclimated Photosystem II.
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DOI：
10.3390/microorganisms10071270
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发表时间：
2022-06-22
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
蓝细菌是全球碳固定的主要贡献者，主要使用可见光（400 - 700 nm）来驱动产氧光合作用。当转移到可见光衰减的环境中时，少量但高度多样化和广泛分布的蓝细菌可以表达修饰的色素和光系统亚基和藻胆蛋白的旁系同源版本，赋予远红光（FRL）吸收（700 - 800 nm），这一过程称为远红光光适应（FaRLiP）。在FaRLiP过程中，交替的光系统II（PSII）亚基使复合物能够结合叶绿素d和f，叶绿素d和f吸收的能量低于叶绿素a，但仍然支持水氧化。FaRLiP反应是如何产生的仍然研究得很少。在这里，我们报告祖先的序列重建和基于结构的分子进化研究的FRL-特异性亚基的FRL-PSII。我们表明，导致两个PsbA（D1）旁系同源物的起源，使叶绿素f和结合叶绿素d在水裂解FRL-PSII的重复很可能是第一次发生之前，现存的蓝藻的多样化。这些重复其次是那些导致替代PsbC（CP43）和PsbD（D2）亚基，发生在早期的蓝藻多样化，并最终与那些导致PsbB（CP47）和PsbH旁系同源物与辐射的主要群体。我们表明，FRL-PSII的起源需要积累相对较少数量的氨基酸变化，祖先FRL-PSII可能包含叶绿素d分子的电子传递链中，两个叶绿素f分子在天线亚基在相同的位置，和三个叶绿素a分子，其网站的能量被改变。结果表明，工程远红光吸收到植物PSII生物技术应用的最小模型。
Cyanobacteria are major contributors to global carbon fixation and primarily use visible light (400−700 nm) to drive oxygenic photosynthesis. When shifted into environments where visible light is attenuated, a small, but highly diverse and widespread number of cyanobacteria can express modified pigments and paralogous versions of photosystem subunits and phycobiliproteins that confer far-red light (FRL) absorbance (700−800 nm), a process termed far-red light photoacclimation, or FaRLiP. During FaRLiP, alternate photosystem II (PSII) subunits enable the complex to bind chlorophylls d and f, which absorb at lower energy than chlorophyll a but still support water oxidation. How the FaRLiP response arose remains poorly studied. Here, we report ancestral sequence reconstruction and structure-based molecular evolutionary studies of the FRL-specific subunits of FRL-PSII. We show that the duplications leading to the origin of two PsbA (D1) paralogs required to make chlorophyll f and to bind chlorophyll d in water-splitting FRL-PSII are likely the first to have occurred prior to the diversification of extant cyanobacteria. These duplications were followed by those leading to alternative PsbC (CP43) and PsbD (D2) subunits, occurring early during the diversification of cyanobacteria, and culminating with those leading to PsbB (CP47) and PsbH paralogs coincident with the radiation of the major groups. We show that the origin of FRL-PSII required the accumulation of a relatively small number of amino acid changes and that the ancestral FRL-PSII likely contained a chlorophyll d molecule in the electron transfer chain, two chlorophyll f molecules in the antenna subunits at equivalent positions, and three chlorophyll a molecules whose site energies were altered. The results suggest a minimal model for engineering far-red light absorbance into plant PSII for biotechnological applications.
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DOI：
10.1073/pnas.2116765118
发表时间：
2022-01-04
影响因子：
11.1
作者：
Gisriel CJ;Wang J;Liu J;Flesher DA;Reiss KM;Huang HL;Yang KR;Armstrong WH;Gunner MR;Batista VS;Debus RJ;Brudvig GW
通讯作者：
Brudvig GW
影响因子：
4.3
作者：
D'Haene, Sandrine E.;Sobotka, Roman;Komenda, Josef
通讯作者：
Komenda, Josef
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
DOI：
10.1016/j.jbc.2021.101424
发表时间：
2022-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gisriel CJ;Shen G;Ho MY;Kurashov V;Flesher DA;Wang J;Armstrong WH;Golbeck JH;Gunner MR;Vinyard DJ;Debus RJ;Brudvig GW;Bryant DA
通讯作者：
Bryant DA
影响因子：
10.7
作者：
Hoang DT;Chernomor O;von Haeseler A;Minh BQ;Vinh LS
通讯作者：
Vinh LS