RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
RbgA ensures the correct timing in the maturation of the 50S subunits functional sites.
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DOI：
10.1093/nar/gkac059
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发表时间：
2022-10-28
影响因子：
14.9
通讯作者：
Ortega, Joaquin
中科院分区：
文献类型：
--
作者：
Seffouh, Amal;Trahan, Chirstian;Wasi, Tanzila;Jain, Nikhil;Basu, Kaustuv;Britton, Robert A.;Oeffinger, Marlene;Ortega, Joaquin
RbgA is an essential protein for the assembly of the 50S subunit in Bacillus subtilis. Depletion of RbgA leads to the accumulation of the 45S intermediate. A strain expressing a RbgA variant with reduced GTPase activity generates spontaneous suppressor mutations in uL6. Each suppressor strain accumulates a unique 44S intermediate. We reasoned that characterizing the structure of these mutant 44S intermediates may explain why RbgA is required to catalyze the folding of the 50S functional sites. We found that in the 44S particles, rRNA helices H42 and H97, near the binding site of uL6, adopt a flexible conformation and allow the central protuberance and functional sites in the mutant 44S particles to mature in any order. Instead, the wild-type 45S particles exhibit a stable H42-H97 interaction and their functional sites always mature last. The dependence on RbgA was also less pronounced in the 44S particles. We concluded that the binding of uL6 pauses the maturation of the functional sites, but the central protuberance continues to fold. RbgA exclusively binds intermediates with a formed central protuberance and licenses the folding of the functional sites. Through this mechanism, RbgA ensures that the functional sites of the 50S mature last. In the Bacillus subtills wild-type strain, the entry of ribosomal protein uL6 pauses the maturation of the functional sites in the assembling subunit, and the central protuberance matures first. Subsequent binding of RbgA unlocks the structure and the A, P and E functional sites mature last. Instead, in the suppressor strains the entry of uL6R3C or uL6R70P variants is unable to pause the maturation of the functional sites, and they assemble ahead of the central protuberance. Maturation of the functional sites in the suppressor strains is also less dependent of RbgA.
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DOI：
10.1107/s2059798318009324
发表时间：
2018-09-01
期刊：
Acta crystallographica. Section D, Structural biology
影响因子：
--
作者：
Afonine PV;Klaholz BP;Moriarty NW;Poon BK;Sobolev OV;Terwilliger TC;Adams PD;Urzhumtsev A
通讯作者：
Urzhumtsev A
影响因子：
4.5
作者：
Gulati M;Jain N;Davis JH;Williamson JR;Britton RA
通讯作者：
Britton RA
影响因子：
64.5
作者：
Davis, Joseph H.;Tan, Yong Zi;Carragher, Bridget;Potter, Clinton S.;Lyumkis, Dmitry;Williamson, James R.
通讯作者：
Williamson, James R.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH