Modular Assembly of the Bacterial Large Ribosomal Subunit.
Modular Assembly of the Bacterial Large Ribosomal Subunit.
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DOI：
10.1016/j.cell.2016.11.020
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发表时间：
2016-12-01
期刊：
影响因子：
64.5
通讯作者：
Williamson, James R.
中科院分区：
文献类型：
--
作者：
Davis, Joseph H.;Tan, Yong Zi;Carragher, Bridget;Potter, Clinton S.;Lyumkis, Dmitry;Williamson, James R.
核糖体是一种复杂的大分子机器，是理解生物学大分子组装的理想系统，因为很少有中间体被隔离并彻底表征了核糖体的核糖体组装。一种必需的核糖体蛋白质，导致组装中间体的积累有能力成熟的质谱。一个组装途径。核糖体组装的景观在体内，并提供了表征核糖体和其他大分子机器的其他组装途径的必要工具。
The ribosome is a complex macromolecular machine and serves as an ideal system for understanding biological macromolecular assembly. Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized. Herein, we deploy a genetic system to starve cells of an essential ribosomal protein, which results in the accumulation of assembly intermediates that are competent for maturation. Quantitative mass spectrometry and single-particle cryo-electron microscopy reveal 13 distinct intermediates, which were each resolved to ~4–5Å resolution and could be placed in an assembly pathway. We find that ribosome biogenesis is a parallel process, that blocks of structured rRNA and proteins assemble cooperatively, and that the entire process is dynamic and can be ‘re-routed’ through different pathways as needed. This work reveals the complex landscape of ribosome assembly in vivo and provides the requisite tools to characterize additional assembly pathways for ribosomes and other macromolecular machines.
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