MR lymphography with iron oxide compound AMI-227: studies in ferrets with filariasis.
MR lymphography with iron oxide compound AMI-227: studies in ferrets with filariasis.
复制标题
使用氧化铁化合物 AMI-227 进行 MR 淋巴成像：对患有丝虫病的雪貂进行研究。
DOI：
10.2214/ajr.159.4.1529857
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. Green
中科院分区：
文献类型：
--
作者：
T. Tanoura;M. Bernas;A. Darkazanli;E. Elam;E. Unger;M. Witte;A. Green
目的
本研究的目的是通过使用雪貂丝虫病动物模型来评估AMI-227降低增生性炎症淋巴结信号强度的MR淋巴造影潜力。研究了间质和IV给药模式。
材料和方法
12只雪貂感染马来丝虫，6只对照雪貂注射超顺磁性氧化铁AMI-227，静脉注射或静脉注射。使用自旋回波和梯度回波技术，在注射造影剂前、注射造影剂后48小时和注射造影剂后138天，测量左腘淋巴结、左腘绳肌和左腹股沟脂肪的信号强度。对信号强度数据进行统计学分析。用光镜和电镜研究淋巴结、肝、脾和肺。
结果
AMI-227组织间注射48小时后，感染和对照雪貂同侧腘淋巴结的信号强度显著降低（p <0.0005），自旋回波和梯度回波MR图像上周围组织的信号强度无显著变化。雪貂静脉注射途径未发生淋巴结信号降低。光学显微镜检查发现铁仅局限于结节边缘区。间质注射后可见淋巴干。信号降低持续到我们的终点138天。
结论
AMI-227显示区域特异性，显著增强淋巴结结构，并显示作为间质MR淋巴造影剂的潜力。
OBJECTIVE
The purpose of this study was to assess the MR lymphographic potential of AMI-227 to reduce the signal intensity of hyperplastic inflammatory lymph nodes by using ferrets with filariasis as the animal model. Both interstitial and IV modes of administration were studied.
MATERIALS AND METHODS
Twelve ferrets were infected with the filaria Brugia malayi and six control ferrets were injected with super-paramagnetic iron oxide AMI-227, either interstitially or IV. Signal intensities of the left popliteal lymph node, left hamstring muscle, and left inguinal fat were measured before, 48 hr after, and up to 138 days after contrast injection with the use of both spin-echo and gradient-echo techniques. The signal intensity data were statistically analyzed. The lymph nodes, liver, spleen, and lungs were studied with light and electron microscopy.
RESULTS
Forty-eight hours after the interstitial injection of AMI-227, the signal intensities of the ipsilateral popliteal nodes of the infected and control ferrets were significantly reduced (p less than .0005) without significant changes in the signal intensities of the surrounding tissues on both spin-echo and gradient-echo MR images. No nodal signal reduction occurred with the IV route of injection in ferrets. Light microscopy revealed iron to be localized within nodal marginal zones exclusively. Lymphatic trunks were visualized after interstitial injection. Signal reduction persisted to our end point at 138 days.
CONCLUSION
AMI-227 shows regional specificity with significant enhancement of nodal structures and demonstrates potential as an interstitial MR lymphographic agent.
影响因子：
19.7
作者：
WEISSLEDER, R;ELIZONDO, G;BRADY, TJ
通讯作者：
BRADY, TJ
慢性感染马来丝虫的雪貂全身淋巴管闪烁扫描。
影响因子：
2.5
作者：
Witte,M;McNeill,G;Crandall,C;Case,T;Witte,C;Crandall,R;Hall,J;Williams,W
通讯作者：
Williams,W