Vegetables affect the expression of genes involved in anticarcinogenic processes in the colonic mucosa of C57BL/6 female mice.
Vegetables affect the expression of genes involved in anticarcinogenic processes in the colonic mucosa of C57BL/6 female mice.
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蔬菜影响 C57BL/6 雌性小鼠结肠粘膜中参与抗癌过程的基因的表达。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
J. V. van Delft
中科院分区：
文献类型：
--
作者：
S. V. van Breda;E. van Agen;S. Van Sanden;T. Burzykowski;A. Kienhuis;J. Kleinjans;J. V. van Delft
有大量的流行病学证据表明，蔬菜消费减少结直肠癌（CRC）的风险。然而，基因组中的分子靶点大多是未知的。本研究采用基因芯片技术研究了蔬菜摄入对雌性C57 B1/6小鼠结肠黏膜基因表达的影响。给小鼠喂食8种饮食之一：不含蔬菜的对照饮食（饮食1）;含100 g/kg（饲料2，10%剂量），200 g/kg（饲料3，20%剂量）或400 g/kg（饮食4，40%剂量）的蔬菜混合物;或含70 g/kg花椰菜的饮食3%剂量）、226 g/kg豌豆（饮食7，22.6%剂量）;或31 g/kg洋葱（饮食8，3.1%剂量）。饮食2至4中使用的蔬菜混合物由饮食5至8中使用的4种单独的蔬菜组成：花椰菜（30%湿重）、胡萝卜（30%湿重）、豌豆（30%湿重）和洋葱（10%湿重）。为了评估基因表达变化，在小鼠被杀死后收集结肠粘膜细胞。提取细胞总RNA，利用基因芯片技术同时检测602个基因的表达水平。对于39个基因，发现了显著的剂量依赖性效应，尽管一般来说，这种关系不是线性的。对于15个基因，改变的表达确实可以解释在CRC发展的各个阶段癌症风险的降低。11个基因被蔬菜混合物以及一种或多种单独的蔬菜调节。对于其中7个基因，混合物的调节是由于特定蔬菜的影响。这些基因特别令人感兴趣，因为它们一直受到影响，并可能参与蔬菜消费预防CRC。
There is abundant epidemiological evidence that vegetable consumption decreases colorectal cancer (CRC) risk. However, the molecular targets in the genome are mostly unknown. The present study investigated the effects of vegetable consumption on gene expression in the colon mucosa of female C57Bl/6 mice using cDNA microarray technology. Mice were fed one of 8 diets: a control diet containing no vegetables (diet 1); a diet containing 100 g/kg (diet 2, 10% dose), 200 g/kg (diet 3, 20% dose), or 400 g/kg (diet 4, 40% dose) of a vegetable mixture; or a diet containing 70 g/kg of cauliflower (diet 5, 7% dose), 73 g/kg of carrots (diet 6, 7.3% dose), 226 g/kg of peas (diet 7, 22.6% dose); or 31 g/kg of onions (diet 8, 3.1% dose). The vegetable mixture used in diets 2 to 4 consisted of the 4 individual vegetables used in diets 5 to 8: cauliflower (30% wet wt), carrots (30% wet wt), peas (30% wet wt), and onions (10% wet wt). To assess gene expression changes, colonic mucosal cells were collected after the mice were killed. Total RNA was isolated and microarray technology was used to measure the expression levels of 602 genes simultaneously. For 39 genes, significant dose-dependent effects were found, although in general the relations were not linear. For 15 genes, the altered expression could indeed explain reduced cancer risk at various stages of CRC development. Eleven genes were modulated by the vegetable mixture as well as by one or more of the individual vegetables. For 7 of the genes, the modulation by the mixture was due to the effect of a particular vegetable. These genes are of particular interest because they were consistently affected and could be involved in the prevention of CRC by vegetable consumption.
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影响因子：
56.9
作者：
Bunz, F;Dutriaux, A;Vogelstein, B
通讯作者：
Vogelstein, B
西兰花补充剂作为谷胱甘肽 S-转移酶活性诱导剂的临床前和临床评估。
DOI：
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发表时间：
1997
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research.
影响因子：
--
作者：
Clapper,ML;Szarka,CE;Pfeiffer,GR;Graham,TA;Balshem,AM;Litwin,S;Goosenberg,EB;Frucht,H;Engstrom,PF
通讯作者：
Engstrom,PF
基因表达微阵列数据的统计设计和分析。
DOI：
10.1017/s0016672301005055
发表时间：
2001
期刊：
Genetical research
影响因子：
--
作者：
Kerr,MK;Churchill,GA
通讯作者：
Churchill,GA
DOI：
10.1016/s0027-5107(01)00187-7
发表时间：
2001-10-01
影响因子：
2.3
作者：
Coles, B;Nowell, SA;Kadlubar, FF
通讯作者：
Kadlubar, FF
影响因子：
4.7
作者：
Mahmoud, NN;Carothers, AM;Bertagnolli, MM
通讯作者：
Bertagnolli, MM