GLT-1 Promoter Activity in Astrocytes and Neurons of Mouse Hippocampus and Somatic Sensory Cortex.
GLT-1 Promoter Activity in Astrocytes and Neurons of Mouse Hippocampus and Somatic Sensory Cortex.
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DOI：
10.3389/neuro.05.031.2009
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发表时间：
2010
影响因子：
2.9
通讯作者：
Conti F
中科院分区：
文献类型：
--
作者：
de Vivo L;Melone M;Rothstein JD;Conti F
采用GLT-1 eGFP BAC报告基因转基因成年小鼠，检测GLT-1基因在CA 1、CA 3和SI细胞中的表达，并通过eGFP荧光定量分析GLT-1启动子在新皮层和海马不同细胞中的活性。几乎所有的GFAP+星形胶质细胞都是eGFP+;我们还发现大约80%的CA 3锥体层神经元，10-70%的SI的I-VI层神经元和CA 1的所有层以及CA 3的定向层和辐射层中的罕见神经元是eGFP+。eGFP强度分析表明，星形胶质细胞有一个更高的GLT-1启动子活性在SI比在CA 1和CA 3，和神经元有最高水平的GLT-1启动子活性在CA 3层的皮层和SI的第VI层。最后，我们观察到神经元中GLT-1启动子活性的强度是星形胶质细胞中测量的强度的1-20%。这些结果表明，在海马和新皮质中，在星形胶质细胞和神经元中观察到GLT-1启动子活性，详细描述了表达GLT-1的神经元的分布，并表明GLT-1启动子在星形胶质细胞和神经元中的活性在不同的脑区域中不同。
GLT-1 eGFP BAC reporter transgenic adult mice were used to detect GLT-1 gene expression in individual cells of CA1, CA3 and SI, and eGFP fluorescence was measured to analyze quantitatively GLT-1 promoter activity in different cells of neocortex and hippocampus. Virtually all GFAP+ astrocytes were eGFP+; we also found that about 80% of neurons in CA3 pyramidal layer, 10–70% of neurons in I-VI layers of SI and rare neurons in all strata of CA1 and in strata oriens and radiatum of CA3 were eGFP+. Analysis of eGFP intensity showed that astrocytes had a higher GLT-1 promoter activity in SI than in CA1 and CA3, and that neurons had the highest levels of GLT-1 promoter activity in CA3 stratum pyramidale and in layer VI of SI. Finally, we observed that the intensity of GLT-1 promoter activity in neurons is 1–20% of that measured in astrocytes. These results showed that in the hippocampus and neocortex GLT-1 promoter activity is observed in astrocytes and neurons, detailed the distribution of GLT-1 expressing neurons, and indicated that GLT-1 promoter activity in both astrocytes and neurons varies in different brain regions.
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影响因子：
4.4
作者：
Hajos, Ferenc
通讯作者：
Hajos, Ferenc
影响因子：
64.8
作者：
KANAI, Y;HEDIGER, MA
通讯作者：
HEDIGER, MA
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作者：
Melone, M;Burette, A;Weinberg, RJ
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Chen, WZ;Mahadomrongkul, V;Rosenberg, PA
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