Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
Mechanosensitive recruitment of stator units promotes binding of the response regulator CheY-P to the flagellar motor.
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DOI：
10.1038/s41467-021-25774-2
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发表时间：
2021-09-14
影响因子：
16.6
通讯作者：
Lele PP
中科院分区：
文献类型：
--
作者：
Antani JD;Gupta R;Lee AH;Rhee KY;Manson MD;Lele PP
细菌鞭毛马达在顺时针(CW)和逆时针(CCW)旋转之间的可逆切换是趋化性所必需的，这使得细胞能够游向有利的化学生境。增加电机旋转的粘性阻力(机械负荷)会抑制开关。然而，细胞在切换到不同粘度的环境中导航时必须保持动态平衡。细胞在不同负荷下维持最佳趋化功能的机制尚不清楚。在这里，我们证明了鞭毛马达通过调节作用在转子上的机械力，变构地控制趋化反应调节剂Chey-P与鞭毛开关复合体的结合亲和力。机械敏感的Chey-P结合通过精确地调整开关对机械刺激的反应来补偿负载引起的开关损失。机械力和Chey-P结合之间的相互作用调节趋化功能以匹配负荷。这种趋化性输出对机械刺激的适应性反应类似于昆虫和脊椎动物神经肌肉系统中的本体感觉反馈。目前尚不清楚细菌细胞如何适应不同粘度的环境中鞭毛马达旋转的可逆切换。在这里，Antani等人。显示鞭毛机械传感器变构控制马达对趋化反应调节剂Chey-P的结合亲和力，以适应不同粘性负荷下的鞭毛切换。
Reversible switching of the bacterial flagellar motor between clockwise (CW) and counterclockwise (CCW) rotation is necessary for chemotaxis, which enables cells to swim towards favorable chemical habitats. Increase in the viscous resistance to the rotation of the motor (mechanical load) inhibits switching. However, cells must maintain homeostasis in switching to navigate within environments of different viscosities. The mechanism by which the cell maintains optimal chemotactic function under varying loads is not understood. Here, we show that the flagellar motor allosterically controls the binding affinity of the chemotaxis response regulator, CheY-P, to the flagellar switch complex by modulating the mechanical forces acting on the rotor. Mechanosensitive CheY-P binding compensates for the load-induced loss of switching by precisely adapting the switch response to a mechanical stimulus. The interplay between mechanical forces and CheY-P binding tunes the chemotactic function to match the load. This adaptive response of the chemotaxis output to mechanical stimuli resembles the proprioceptive feedback in the neuromuscular systems of insects and vertebrates. It is unclear how bacterial cells adapt the reversible switching of flagellar motor rotation to environments of different viscosities. Here, Antani et al. show that flagellar mechanosensors allosterically control the motor’s binding affinity for the chemotaxis response regulator, CheY-P, to adapt flagellar switching over varying viscous loads.
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驱动细菌鞭毛旋转的定子配合物的结构。
影响因子：
28.3
作者：
Deme JC;Johnson S;Vickery O;Aron A;Monkhouse H;Griffiths T;James RH;Berks BC;Coulton JW;Stansfeld PJ;Lea SM
通讯作者：
Lea SM
DOI：
10.2142/biophysics.9.173
发表时间：
2013
期刊：
Biophysics (Nagoya-shi, Japan)
影响因子：
--
作者：
Castillo DJ;Nakamura S;Morimoto YV;Che YS;Kami-Ike N;Kudo S;Minamino T;Namba K
通讯作者：
Namba K
影响因子：
6.4
作者：
Tipping MJ;Delalez NJ;Lim R;Berry RM;Armitage JP
通讯作者：
Armitage JP
影响因子：
3.6
作者：
Sourjik, V;Berg, HC
通讯作者：
Berg, HC
DOI：
10.1073/pnas.1716002114
发表时间：
2017-12-05
影响因子：
11.1
作者：
Nord AL;Gachon E;Perez-Carrasco R;Nirody JA;Barducci A;Berry RM;Pedaci F
通讯作者：
Pedaci F