XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure.
XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure.
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DOI：
10.1083/jcb.132.3.259
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发表时间：
1996-02
影响因子：
7.8
通讯作者：
Lawrence, JB
中科院分区：
文献类型：
--
作者：
Clemson, CM;McNeil, JA;Willard, HF;Lawrence, JB
XIST 基因与 X 染色体失活有关，但 RNA 不包含明显的开放阅读框。在其转录位点（无活性的 X 染色体 (Xi)）附近观察到 XIST RNA 的积累。一系列分子细胞遗传学研究比较了 XIST RNA 与其他蛋白质编码 RNA 的特性，支持了 XIST RNA 的关键区别； XIST 并不仅仅集中在 Xi，因为它是在那里转录和处理的。最值得注意的是，形态测量和 3D 分析表明 XIST RNA 和 Xi 在 2D 和 3D 空间中是一致的；因此，XIST RNA 本质上描绘了 Xi。多项结果表明，XIST RNA 积累有两种成分，一种是与转录和加工相关的次要成分，另一种是与 Xi 稳定结合的剪接主要成分。转录抑制后，主要剪接成分保留在细胞核中，并经常包围特别突出的异染色质巴尔小体。持续转录的 XIST 基因及其聚腺苷酸化 RNA 始终定位于缺乏剪接因子/聚 A RNA 丰富结构域的核区域。去除染色体 DNA 后，XIST RNA 仍保留在核基质部分中。 XIST RNA 在有丝分裂期间从与 Xi 的结合中释放出来，但显示出独特的高度颗粒分布。集体结果表明，XIST RNA 可能是间期染色体区域的结构元素，也可能是与 Xi 特异性相关的非染色质核结构的组成部分。 XIST RNA 是一种新型核 RNA，它可能为 RNA 通过高阶染色质包装参与核结构和顺式限制基因调控提供特定的先例。
The XIST gene is implicated in X chromosome inactivation, yet the RNA contains no apparent open reading frame. An accumulation of XIST RNA is observed near its site of transcription, the inactive X chromosome (Xi). A series of molecular cytogenetic studies comparing properties of XIST RNA to other protein coding RNAs, support a critical distinction for XIST RNA; XIST does not concentrate at Xi simply because it is transcribed and processed there. Most notably, morphometric and 3-D analysis reveals that XIST RNA and Xi are coincident in 2- and 3-D space; hence, the XIST RNA essentially paints Xi. Several results indicate that the XIST RNA accumulation has two components, a minor one associated with transcription and processing, and a spliced major component, which stably associates with Xi. Upon transcriptional inhibition the major spliced component remains in the nucleus and often encircles the extra-prominent heterochromatic Barr body. The continually transcribed XIST gene and its polyadenylated RNA consistently localize to a nuclear region devoid of splicing factor/poly A RNA rich domains. XIST RNA remains with the nuclear matrix fraction after removal of chromosomal DNA. XIST RNA is released from its association with Xi during mitosis, but shows a unique highly particulate distribution. Collective results indicate that XIST RNA may be an architectural element of the interphase chromosome territory, possibly a component of nonchromatin nuclear structure that specifically associates with Xi. XIST RNA is a novel nuclear RNA which potentially provides a specific precedent for RNA involvement in nuclear structure and cis-limited gene regulation via higher-order chromatin packaging.
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poly（a）RNA的离散核域及其与核的功能组织的关系。
影响因子：
7.8
作者：
Carter, K C;Taneja, K L;Lawrence, J B
通讯作者：
Lawrence, J B
DOI：
10.1083/jcb.127.3.593
发表时间：
1994-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Blencowe BJ;Nickerson JA;Issner R;Penman S;Sharp PA
通讯作者：
Sharp PA
DOI：
10.1083/jcb.129.2.287
发表时间：
1995-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bregman DB;Du L;van der Zee S;Warren SL
通讯作者：
Warren SL
DOI：
10.1101/sqb.1993.058.01.085
发表时间：
1993-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CREMER, T;KURZ, A;LICHTER, P
通讯作者：
LICHTER, P
影响因子：
64.5
作者：
CHUANG, PT;ALBERTSON, DG;MEYER, BJ
通讯作者：
MEYER, BJ