Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics.
Exosomes from Human Umbilical Cord Mesenchymal Stem Cells: Identification, Purification, and Biological Characteristics.
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人脐带间充质干细胞外泌体：鉴定、纯化和生物学特性
DOI：
10.1155/2016/1929536
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发表时间：
2016
影响因子：
4.3
通讯作者：
Xu W
中科院分区：
文献类型：
--
作者：
Zhang B;Shen L;Shi H;Pan Z;Wu L;Yan Y;Zhang X;Mao F;Qian H;Xu W
我们和其他研究小组已经发现，间充质干细胞（MSC）衍生的外泌体是许多疾病的一种新的治疗方式。本研究总结了本实验室采用超滤和梯度离心法提取和纯化hucMSCs-exosomes的方法，并证明了该方法制备的hucMSCs-exosomes具有稳定性和生物活性。结果显示hucMSC来源的exosomes为40~100 nm，CD 9和CD 81阳性。在功能上，hucMSC-外泌体通过激活ERK 1/2和p38促进细胞增殖并在体外保护免受氧化应激诱导的细胞凋亡。有趣的是，紫外线暴露消除了外泌体在氧化应激下的调节作用，表明hucMSCs-exosomes可以通过RNA的外泌体穿梭来调节细胞生长和凋亡。此外，细胞因子谱分析显示hucMSC-外泌体含有高剂量的IL-6、IL-8和其他细胞因子。所建立的方法实用、高效，为进一步评价hucMSCs-exosomes作为治疗药物的潜力奠定了基础。
Our and other groups have discovered that mesenchymal stem cells (MSCs) derived exosomes are a novel therapeutical modality for many diseases. In this study, we summarized a method to extract and purify hucMSCs-exosomes using ultrafiltration and gradient centrifugation in our laboratory and proved that hucMSCs-exosomes prepared according to our procedure were stable and bioactive. Results showed that exosomes derived from hucMSC were 40~100 nm and CD9 and CD81 positive. Functionally, hucMSCs-exosomes promoted cell proliferation and protected against oxidative stress-induced cell apoptosis in vitro by activation of ERK1/2 and p38. Interestingly, UV exposure abrogated the regulatory roles of exosomes under oxidative stress, indicating that hucMSCs-exosomes may regulate cell growth and apoptosis by exosomal shuttle of RNA. Furthermore, cytokine profile analysis revealed that hucMSCs-exosomes contained high dose of IL-6, IL-8, and other cytokines. The established method is practical and efficient, which provides a basis for further evaluating the potential of hucMSCs-exosomes as therapeutic agents.
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影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
5.8
作者：
Chaput, N;Taïeb, J;Zitvogel, L
通讯作者：
Zitvogel, L
间充质基质细胞来源的外泌体的免疫调节作用
影响因子：
4.4
作者：
Chen, Wancheng;Huang, Yukai;Xiao, Yang
通讯作者：
Xiao, Yang
影响因子：
6
作者：
Doeppner, Thorsten R.;Herz, Josephine;Hermann, Dirk M.
通讯作者：
Hermann, Dirk M.
DOI：
10.1083/jcb.101.3.942
发表时间：
1985-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Pan BT;Teng K;Wu C;Adam M;Johnstone RM
通讯作者：
Johnstone RM