Prevention of Nonalcoholic Hepatic Steatosis by Shenling Baizhu Powder: Involvement of Adiponectin-Induced Inhibition of Hepatic SREBP-1c.
Prevention of Nonalcoholic Hepatic Steatosis by Shenling Baizhu Powder: Involvement of Adiponectin-Induced Inhibition of Hepatic SREBP-1c.
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参苓白术散预防非酒精性肝脂肪变性：参与脂联素诱导的肝脏 SREBP-1c 抑制
DOI：
10.1155/2020/9701285
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发表时间：
2020
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Tang K;Deng Y;Zheng C;Nie H;Pan M;Chen R;Xie J;Yang Q;Zhang Y
背景非酒精性脂肪性肝病（nonalcoholic fatty liver disease，NAFLD）是世界范围内常见的慢性肝病，其发病率呈逐年上升趋势，但目前尚无治疗NAFLD的特效药物。参苓白术散是临床常用的安全中药复方。我们前期的研究表明SL具有预防NAFLD的作用，但其具体机制尚未确定。本研究通过动物实验探讨了SL治疗NAFLD的可能机制。方法Wistar大鼠喂饲胆碱缺乏的氨基酸限定饲料（CDAA）8周后给予SL。然后采集血清样本获取生化指标;采集脂肪组织和肝脏样本进行病理检测;采用moorFLPI-2血流成像仪测量肝脏微循环血流量;采用大鼠细胞因子芯片筛选潜在靶蛋白。Western blotting检测肝脂联素/SREBP-1c通路相关蛋白的表达。结果SL能有效降低CDAA诱导的大鼠肝湿重，降低肝脏总胆固醇（TC）和甘油三酯（TG）水平，改善肝损伤。病理学检查显示，SL能明显减少肝脏脂滴，改善肝脏脂质蓄积。此外，肝血流量检测显示，SL增加CDAA喂养大鼠的肝脏微循环。通过细胞因子芯片，在肝脏中筛选差异表达的细胞因子，即脂联素。Western blotting结果显示，SL可增加肝脏脂联素和磷酸乙酰辅酶A羧化酶（p-ACC）的表达，降低类固醇调节元件结合蛋白-1c（SREBP-1c）和脂肪酸合成酶（FAS）的表达。结论SL可提高肝脏和血清脂联素水平，抑制SREBP-1c表达，从而调节全身脂质代谢，减少肝脏脂质蓄积。
Background Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease worldwide, and its incidence is increasing annually, but there is currently no specific drug for treating NAFLD. Shenling Baizhu powder (SL) is a safe herbal compound commonly used in clinical practice. Our previous research has shown that SL has the effect of preventing NAFLD, but its specific mechanism has not been determined. In this study, the potential mechanism of SL on NAFLD was explored by in vivo experiments. Methods Wistar rats fed a choline-deficient amino acid-defined diet (CDAA) were treated with SL for 8 weeks. Then, serum samples were collected to obtain biochemical indicators; adipose tissue and liver samples were collected for pathological detection; a moorFLPI-2 blood flow imager was used to measure liver microcirculation blood flow, and a rat cytokine array was used to screen potential target proteins. The expression of liver adiponectin/SREBP-1c pathway-related proteins was determined by Western blotting. Results SL effectively reduced the liver wet weight, as well as the levels of total cholesterol (TC) and triglyceride (TG) in the liver, and ameliorated liver injury in CDAA-fed rats. Pathological examinations showed that SL markedly reduced liver lipid droplets and improved liver lipid accumulation. In addition, the detection of liver blood flow showed that SL increased liver microcirculation in CDAA-fed rats. Through the cytokine array, a differentially expressed cytokine, namely, adiponectin, was screened in the liver. Western blotting assays showed that SL increased the expression of adiponectin and phosphoacetyl-CoA Carboxylase (p-ACC) in the liver and decreased the expression of steroid regulatory element-binding protein-1c (SREBP-1c) and fatty acid synthase (FAS). Conclusion These results suggest that SL can increase the levels of adiponectin in the liver and serum and can inhibit the expression of SREBP-1c, thereby regulating systemic lipid metabolism and reducing liver lipid accumulation.
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影响因子：
4.6
作者：
Lovric A;Granér M;Bjornson E;Arif M;Benfeitas R;Nyman K;Ståhlman M;Pentikäinen MO;Lundbom J;Hakkarainen A;Sirén R;Nieminen MS;Lundbom N;Lauerma K;Taskinen MR;Mardinoglu A;Boren J
通讯作者：
Boren J
中药提取物诱导自噬作为治疗非酒精性脂肪肝的新方法
影响因子：
4.3
作者：
Liu C;Liao JZ;Li PY
通讯作者：
Li PY
影响因子：
82.9
作者：
Friedman SL;Neuschwander-Tetri BA;Rinella M;Sanyal AJ
通讯作者：
Sanyal AJ
DOI：
10.1016/j.jsbmb.2014.02.009
发表时间：
2014-09-01
影响因子：
4.1
作者：
Li, Xinwei;Li, Yu;Li, Xiaobing
通讯作者：
Li, Xiaobing
影响因子：
9.8
作者：
Buzzetti, Elena;Pinzani, Massimo;Tsochatzis, Emmanuel A.
通讯作者：
Tsochatzis, Emmanuel A.