Effect of low daily doses of mifepristone on ovarian function and endometrial development.
Effect of low daily doses of mifepristone on ovarian function and endometrial development.
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低日剂量米非司酮对卵巢功能和子宫内膜发育的影响。
DOI：
10.1093/humrep/11.2.256
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发表时间：
1996
影响因子：
6.1
通讯作者：
M. Seppälä
中科院分区：
文献类型：
--
作者：
K. Gemzell‐Danielsson;P. Westlund;E. Johannisson;M. Swahn;M. Bygdeman;M. Seppälä
研究了每日低剂量的抗孕激素米非司酮 (RU 486) 对卵巢和子宫内膜功能的影响。该研究包括一个对照周期、三个治疗周期和一个随访周期。在治疗周期中，每日一次给予 0.1 (n = 5) 或 0.5 (n = 5) mg 米非司酮。在对照和治疗周期期间每周收集三次尿液样本，并通过放射免疫测定法对孕二醇葡萄糖醛酸和雌酮葡萄糖醛酸以及黄体生成素（LH）进行定量。每周采集一次用于皮质醇测量的血样，并在月经开始时采集血样用于测定血清甘油三酯。在对照周期的黄体中期以及第一和第三治疗周期中进行子宫内膜活检，并通过形态测定和组织化学方法进行分析。测量了双花豆凝集素（DBA）凝集素的结合，并通过免疫组织化学分析了孕酮和雌激素受体以及甘氨肽的表达。研究的所有周期均为排卵期，具有 LH 峰值和孕二醇葡萄糖苷酸浓度升高。通过超声检查判断卵泡发育似乎正常。月经周期的长度和月经出血量没有显着改变。每天服用 0.5 mg 米非司酮后，子宫内膜发育似乎略有延迟，腺体直径显着减小。此外，还观察到 DBA 凝集素结合和子宫内膜糖皮质激素表达显着降低。每日剂量0.1毫克对子宫内膜没有任何显着影响。对照组和治疗周期之间的雌激素或孕激素受体免疫活性没有差异。这项研究进一步证明，即使抗孕激素剂量足够低，不会干扰排卵，子宫内膜功能也很敏感。这些效应是否足以阻止植入仍有待确定。
The effects of low daily doses of the antiprogestin mifepristone (RU 486) on ovarian and endometrial function were studied. The study included one control cycle, three treatment cycles and one follow-up cycle. During the treatment cycles, either 0.1 (n = 5) or 0.5 (n = 5) mg of mifepristone was administered once daily. Urine samples were collected three times weekly during the control and treatment cycles and pregnanediol glucuronide and oestrone glucuronide and luteinizing hormone (LH) were quantified by radioimmunoassay. Blood samples for cortisol measurement were collected once weekly and for serum glycodelin at the onset of menstruation. An endometrial biopsy was obtained in the mid-luteal phase in the control cycle and in the first and third treatment cycles and analysed by morphometric and histochemical methods. Binding of Dolichus biflorus agglutinin (DBA) lectin was measured and expression of progesterone and oestrogen receptors and glycodelin were analysed immunohistochemically. All cycles studied were ovulatory with an LH peak and elevated pregnanediol glucuronide concentrations. Follicular development seemed normal as judged by ultrasound examination. The length of the menstrual cycle and the menstrual bleeding were not significantly altered. Following administration of 0.5 mg mifepristone/day, endometrial development appeared to be slightly retarded and glandular diameter was significantly reduced. Furthermore, significant decreases in DBA lectin binding and endometrial expression of glycodelin were observed. Daily doses of 0.1 mg did not have any significant effect on the endometrium. No differences in oestrogen or progesterone receptor immunoactivity between control and treatment cycles were seen. This study provides further evidence that endometrial function is sensitive even to doses of antiprogestin that are low enough not to disturb ovulation. It remains to be established whether these effects are sufficient to prevent implantation.
DOI：
10.1016/s0002-9378(87)80236-3
发表时间：
1987-12-01
影响因子：
9.8
作者：
SHOUPE, D;MISHELL, DR;LOBO, RA
通讯作者：
LOBO, RA