ENRICHMENT OF IN VIVO EFFICACY OF CATECHIN RICH EXTRACT WITH THE APPLICATION OF NANOTECHNOLOGY
ENRICHMENT OF IN VIVO EFFICACY OF CATECHIN RICH EXTRACT WITH THE APPLICATION OF NANOTECHNOLOGY
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纳米技术应用增强儿茶素丰富提取物的体内功效
DOI：
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
S. Bharath
中科院分区：
文献类型：
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作者：
P. Monika;B. Basavaraj;K. Murthy;N. Ahalya;S. Bharath
目的：本研究的主要目标是利用可生物降解且无毒的聚合物Eudragit L 100将富含儿茶素的天然提取物转化为纳米颗粒，以解决儿茶素的各种生物制药问题。方法：使用Eudragit L 100 浓度不断增加，通过乳液溶剂蒸发技术制备纳米颗粒。适当尝试优化加工条件，如有机溶剂、稀释剂和表面活性剂浓度、药物和聚合物比例以及干燥方法的选择，以提高生物效率。评估动态光散射、zeta 电位、SEM 和能量色散 X 射线光谱等参数来评估纳米颗粒。结果：与其他有机溶剂相比，甲醇的包封率在 35-45% 之间。所有制剂的zeta电位值均在±30 mV至±60 mV的范围内，这证实了中等至良好的稳定性。药物在 pH 6.8 缓冲液中的快速或“爆发”释放效果在前 30 分钟内显示为 92%，到 180 分钟结束时逐渐下降至 52%，但在 pH 7.4 中，发现释放是中等的。 SEM 和 DLS 表明颗粒呈球形，纳米范围为 100 至 200 nm，聚合物对颗粒尺寸的影响成比例。结论：发现纳米制剂更加稳定，并证实了碳和氧等主要元素的存在。这些发现共同表明，应用纳米技术来设计先进的口服剂型以提高生物利用度和生物功效可能是值得的。
Objective: The primary goal of this study was to convert a natural catechin-rich extract into nanoparticles by using a biodegradable and non-toxic polymer Eudragit L 100 to address the various biopharmaceutical problems of catechin. Methods: Nanoparticles were prepared by emulsion solvent evaporation technique using Eudragit L 100 in increasing concentration. Optimization of processing conditions like a selection of organic solvents, diluent and surfactant concentrations, drug and polymer ratio and method of drying to increase the biological efficiency were duly attempted. Parameters such as dynamic light scattering, zeta potential, SEM and energy-dispersive X-ray spectroscopy were assessed for the evaluation of nanoparticles. Results: The entrapment efficiency was found to be between 35-45 % with methanol compared to other organic solvents. The zeta potential values of all the formulations were in the range of±30 mV to±60 mV) which confirms moderate to good stability. A rapid or ‘burst’ effect of the drug release in pH 6.8 buffer showing 92 % in the first 30 min which gradually decreased to 52 % by the end of 180 min but in the pH 7.4, the release was found to be moderate. SEM and DLS indicated particles were of spherical shape lying in a nanometer range of 100 to 200 nm with a proportional influence of polymer on the particles size. Conclusion: Nanoformulations were found to be more stable and confirmed the presence of major elements such as carbon and oxygen. The findings collectively indicate that it may be worthwhile to apply nanotechnology for the design of an advanced oral dosage form for an enhanced bioavailability and biological efficacy.
影响因子：
9.7
作者：
Sanna, Vanna;Siddiqui, Imtiaz A.;Sechi, Mario;Mukhtar, Hasan
通讯作者：
Mukhtar, Hasan